Anti-Antithrombin III/ATIII antibody [1 B7] (Biotin) (ab197585)

Overview

  • Product name

    Anti-Antithrombin III/ATIII antibody [1 B7] (Biotin)
    See all Antithrombin III/ATIII primary antibodies
  • Description

    Mouse monoclonal [1 B7] to Antithrombin III/ATIII (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Full length native protein (purified) corresponding to Human Antithrombin III/ATIII aa 33-464. (From Human plasma).
    Sequence:

    HGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWE LSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQ QLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDK SLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITD VIPSEAINELTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESCSAS MMYQEGKFRYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEKELT PEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSK LPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRV TFKANRPFLVFIREVPLNTIIFMGRVANPCVK


    Database link: P01008

  • General notes

    Previously labelled as Antithrombin III.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.0975% Sodium azide
    Constituents: 0.81% Sodium chloride, 99% Phosphate Buffer
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1 B7
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [22] to Thrombin-Antithrombin Complex (ab191378).

ab197585 reacts strongly with native Antithrombin III / ATIII. Strong reaction is seen in ELISA with native Antithrombin III / ATIII coated directly onto the microtiter well. In a sandwich ELISA setup, ab197585 acts as a detection antibody in combination with ab191378 as capture antibody to measure Thrombin-Antithrombin (TAT) complexes. 

Target

  • Function

    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificity

    Found in plasma.
  • Involvement in disease

    Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin family C member 1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

Images

  • Calibration curve of a Sandwich ELISA for TAT (Thrombin-Antithrombin) using ab191378 as capture antibody and ab197585 as detection antibody.

References

ab197585 has not yet been referenced specifically in any publications.

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