Overview

  • Product name

    Anti-Antithrombin III/ATIII antibody
    See all Antithrombin III/ATIII primary antibodies
  • Description

    Sheep polyclonal to Antithrombin III/ATIII
  • Host species

    Sheep
  • Tested applications

    Suitable for: IHC-P, Immunodiffusion, Immunoelectrophoresis, Double Immunodiffusionmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cat, DogDoes not react with: Rabbit, Goat, Chicken, Guinea pig, Cow, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Human Antithrombin III/ATIII.

  • Positive control

    • Human intestine tissue
  • General notes

    Reactivity against the sera of the listed species has been assessed by double diffusion using 10 ul of antisera against 1 ul of species sera.

    Previously labelled as Antithrombin III.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 99% Glycine buffered saline, 0.1% EACA, 0.01% Benzamidine, 0.03% EDTA
  • Concentration information loading...
  • Purity

    DEAE-Chromatography
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Immunodiffusion Use at an assay dependent dilution. RID: 6 ul antiserum/cm2 gel vs. 5 ul neat -1:10 dilution human plasma.
Immunoelectrophoresis Use at an assay dependent dilution. 100 ul antiserum vs. 5 ul plasma gives single arc. Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
Double Immunodiffusion Use at an assay dependent dilution. 10 ul antiserum vs. 5 ul plasma.

Target

  • Function

    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificity

    Found in plasma.
  • Involvement in disease

    Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin family C member 1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

Images

  • ab124259, at 5µg/ml, staining Antithrombin III/ATIII in formalin-fixed, paraffin-embedded Human intestine vessels tissue.

References

ab124259 has not yet been referenced specifically in any publications.

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