Key features and details
- Sheep polyclonal to Antithrombin III/ATIII
- Suitable for: IHC-P, Immunodiffusion, Immunoelectrophoresis, Double Immunodiffusion
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Antithrombin III/ATIII antibody
See all Antithrombin III/ATIII primary antibodies
DescriptionSheep polyclonal to Antithrombin III/ATIII
Tested applicationsSuitable for: IHC-P, Immunodiffusion, Immunoelectrophoresis, Double Immunodiffusionmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cat, DogDoes not react with: Rabbit, Goat, Chicken, Guinea pig, Cow, Pig
Full length native protein (purified) corresponding to Human Antithrombin III/ATIII.
- Human intestine tissue
General notesReactivity against the sera of the listed species has been assessed by double diffusion using 10 ul of antisera against 1 ul of species sera.
Previously labelled as Antithrombin III.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.1% EACA, 0.01% Benzamidine, 0.03% EDTA, 99% Glycine buffered saline
Concentration information loading...
Our Abpromise guarantee covers the use of ab124259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|Immunodiffusion||Use at an assay dependent dilution. RID: 6 ul antiserum/cm2 gel vs. 5 ul neat -1:10 dilution human plasma.|
|Immunoelectrophoresis||Use at an assay dependent dilution. 100 ul antiserum vs. 5 ul plasma gives single arc. Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.|
|Double Immunodiffusion||Use at an assay dependent dilution. 10 ul antiserum vs. 5 ul plasma.|
FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Tissue specificityFound in plasma.
Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
Sequence similaritiesBelongs to the serpin family.
modificationsPhosphorylation sites are present in the extracelllular medium.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- ANT3_HUMAN antibody
- Antithrombin antibody
- Antithrombin III antibody
ab124259 has not yet been referenced specifically in any publications.