Key features and details
- Rabbit polyclonal to Antithrombin III/ATIII
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Antithrombin III/ATIII antibody
See all Antithrombin III/ATIII primary antibodies
DescriptionRabbit polyclonal to Antithrombin III/ATIII
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Orangutan
- U251 cell extract.
Previously labelled as Antithrombin III.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 52 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Tissue specificityFound in plasma.
Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
Sequence similaritiesBelongs to the serpin family.
modificationsPhosphorylation sites are present in the extracelllular medium.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- ANT3_HUMAN antibody
- Antithrombin antibody
- Antithrombin III antibody
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab180614. Blue DAPI for nuclear staining.
All lanes : Anti-Antithrombin III/ATIII antibody (ab180614) at 1/500 dilution
Lane 1 : U251 cell extract
Lane 2 : HepG2 cell extract
Lane 3 : PC3 cell extract
Lane 4 : Ho8910 cell extract
Lane 5 : Mouse liver extract
Predicted band size: 52 kDa
ab180614 has been referenced in 1 publication.
- Iba T et al. The Comparison of the Protective Effects of a- and ß-Antithrombin against Vascular Endothelial Cell Damage Induced by Histone in Vitro. TH Open 1:e3-e10 (2017). PubMed: 31249909