Key features and details
- Rabbit polyclonal to Antithrombin III/ATIII
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Antithrombin III/ATIII antibody
See all Antithrombin III/ATIII primary antibodies
DescriptionRabbit polyclonal to Antithrombin III/ATIII
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse
Recombinant full length protein (His-tag) corresponding to Rat Antithrombin III/ATIII aa 33-465. (Expressed in E.coli).
HGNPVDDICIAKPRDIPVNPMCIYRSPAKKATEEDVLEQKVPEATNRRVW ELSKANSRFATNFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNNTL KQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSNLVSANRLFGD KSLTFNESYQDVSEIVYGAKLQPLDFKENPEQSRVTINNWVANKTEGRIK DVIPQGAIDELTALVLVNTIYFKGLWKSKFSPENTRKEPFHKVDGQSCLV PMMYQEGKFKYRRVGEGTQVLEMPFKGDDITMVLILPKPEKSLAKVEQEL TPELLQEWLDELSEVMLVVHVPRFRIEDSFSLKEQLQDMGLVDLFSPEKS QLPGIIAEGRDDLFVSDAFHKAFLEVNEEGSEAAASTSVVITGRSLNPSR VTFKANRPFLVLIREVALNTIIFMGRVSNPCVN
- IHC-P: Rat liver, kidney, small intestine and heart tissues. WB: Native rat Antithrombin III/ATIII protein (ab93317), Rat serum; Rat heart and liver lysates; Recombinant rat Antithrombin III/ATIII protein.
Previously labelled as Antithrombin III.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231005 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 52 kDa.|
FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Tissue specificityFound in plasma.
Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
Sequence similaritiesBelongs to the serpin family.
modificationsPhosphorylation sites are present in the extracelllular medium.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- ANT3_HUMAN antibody
- Antithrombin antibody
- Antithrombin III antibody
Paraffin-embedded rat heart tissue stained for Antithrombin lll / ATIII using ab231005 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
Anti-Antithrombin III/ATIII antibody (ab231005) at 2 µg/ml + Recombinant rat Antithrombin lll / ATIII protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 52 kDa
All lanes : Anti-Antithrombin III/ATIII antibody (ab231005) at 2 µg/ml
Lane 1 : Rat serum
Lane 2 : Rat heart lysate
Lane 3 : Rat liver lysate
Lane 4 : Rat lung lysate
Lane 5 : Rat brain lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Paraffin-embedded rat small intestine tissue stained for Antithrombin lll / ATIII using ab231005 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
Paraffin-embedded rat kidney tissue stained for Antithrombin lll / ATIII using ab231005 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
Paraffin-embedded rat liver tissue stained for Antithrombin lll / ATIII using ab231005 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231005 has not yet been referenced specifically in any publications.