Overview

  • Product name

    Anti-Antithrombin III/ATIII antibody
    See all Antithrombin III/ATIII primary antibodies
  • Description

    Rabbit polyclonal to Antithrombin III/ATIII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Rat Antithrombin III/ATIII aa 33-465. (Expressed in E.coli).
    Sequence:

    HGNPVDDICIAKPRDIPVNPMCIYRSPAKKATEEDVLEQKVPEATNRRVW ELSKANSRFATNFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNNTL KQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSNLVSANRLFGD KSLTFNESYQDVSEIVYGAKLQPLDFKENPEQSRVTINNWVANKTEGRIK DVIPQGAIDELTALVLVNTIYFKGLWKSKFSPENTRKEPFHKVDGQSCLV PMMYQEGKFKYRRVGEGTQVLEMPFKGDDITMVLILPKPEKSLAKVEQEL TPELLQEWLDELSEVMLVVHVPRFRIEDSFSLKEQLQDMGLVDLFSPEKS QLPGIIAEGRDDLFVSDAFHKAFLEVNEEGSEAAASTSVVITGRSLNPSR VTFKANRPFLVLIREVALNTIIFMGRVSNPCVN

  • Positive control

    • IHC-P: Rat liver, kidney, small intestine and heart tissues. WB: Native rat Antithrombin III/ATIII protein (ab93317), Rat serum; Rat heart and liver lysates; Recombinant rat Antithrombin III/ATIII protein.
  • General notes

    Previously labelled as Antithrombin III.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab231005 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 52 kDa.

Target

  • Function

    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificity

    Found in plasma.
  • Involvement in disease

    Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin family C member 1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

Images

  • Paraffin-embedded rat heart tissue stained for Antithrombin lll / ATIII using ab231005 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.

  • Anti-Antithrombin III/ATIII antibody (ab231005) at 2 µg/ml + Recombinant rat Antithrombin lll / ATIII protein

    Secondary
    HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

  • All lanes : Anti-Antithrombin III/ATIII antibody (ab231005) at 2 µg/ml

    Lane 1 : Rat serum
    Lane 2 : Rat heart lysate
    Lane 3 : Rat liver lysate
    Lane 4 : Rat lung lysate
    Lane 5 : Rat brain lysate

    Secondary
    All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

  • Paraffin-embedded rat small intestine tissue stained for Antithrombin lll / ATIII using ab231005 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.

  • Paraffin-embedded rat kidney tissue stained for Antithrombin lll / ATIII using ab231005 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.

  • Paraffin-embedded rat liver tissue stained for Antithrombin lll / ATIII using ab231005 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.

References

ab231005 has not yet been referenced specifically in any publications.

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