Overview

  • Product name

    Anti-Antithrombin III/ATIII antibody
    See all Antithrombin III/ATIII primary antibodies
  • Description

    Sheep polyclonal to Antithrombin III/ATIII
  • Host species

    Sheep
  • Tested applications

    Suitable for: Double Immunodiffusion, RID, Immunoelectrophoresismore details
  • Species reactivity

    Reacts with: Mouse, Rat, Horse, Cat, Dog, Human
    Does not react with: Rabbit, Chicken, Guinea pig, Cow, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Human Antithrombin III/ATIII. Purified from Plasma

  • General notes

    The concentration varies from batch to batch (10-20 mg/mL).

    Previously labelled as Antithrombin III.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.1% EACA, 0.01% Benzamidine, 0.0292% EDTA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Antiserum is prepared by immunisation of sheep with Antithrombin III/ATIII and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2µm filtered.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Double Immunodiffusion Use at an assay dependent concentration.
RID Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.

Target

  • Function

    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificity

    Found in plasma.
  • Involvement in disease

    Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin family C member 1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

References

ab8776 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I have received the following protocol for ab13335 in direct ELISA (not sandwich as sandwich ELISA has not been tested with ab13335) which I hope will help you. 1. Coating 1-5 ug/ml per well of Anti-thrombin III on High binding plates. Incubation for overnight at 4C. 2. Blocking with 1% BSA in PBS for 30 minutes. 3. Wash buffer: PBS Tween 0.05%. 4. Primary antibody: this product at 1:1,000-1:2,000 dilutions. Incubation for 120 minutes at room temperature. 5. Secondary antibody: HRP APA mouse IgG at 1:500-1:1,000 dilutions. Incubation for 120 minutes at room temperature. 6. Substrate: OPD Please let me know if this helps. Best of luck with your research

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Answer

Thank you for your email. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any additional questions, please contact us again.

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Question
Answer

Thank you for your enquiry. We are sorry to inform you that there is no protein concentration for polyclonal antibodies that are not affinity purified. If you have any more questions, please contact us again.

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