Overview

  • Product name

    Anti-Antithrombin III/ATIII antibody [EP5372]
    See all Antithrombin III/ATIII primary antibodies
  • Description

    Rabbit monoclonal [EP5372] to Antithrombin III/ATIII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Antithrombin III/ATIII aa 350-450.

  • Positive control

    • Human plasma, Human ovary cancer, Human testis and fetal lung lysates. Human liver tissue.
  • General notes

    Previously labelled as Antithrombin III.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.26 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP5372
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
    • Tissue specificity

      Found in plasma.
    • Involvement in disease

      Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
    • Sequence similarities

      Belongs to the serpin family.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted > extracellular space.
    • Information by UniProt
    • Database links

    • Alternative names

      • ANT3_HUMAN antibody
      • Antithrombin antibody
      • Antithrombin III antibody
      • Antithrombin-III antibody
      • AntithrombinIII antibody
      • AT 3 antibody
      • AT III antibody
      • AT3 antibody
      • AT3D antibody
      • ATIII antibody
      • Heparin cofactor I antibody
      • MGC22579 antibody
      • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
      • Serine cysteine proteinase inhibitor clade C member 1 antibody
      • Serine proteinase inhibitor clade C member 1 antibody
      • Serpin C1 antibody
      • Serpin family C member 1 antibody
      • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
      • SERPINC1 antibody
      • THPH7 antibody
      see all

    Images

    • All lanes : Anti-Antithrombin III/ATIII antibody [EP5372] (ab126598) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : Human testis lysate
      Lane 4 : Fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?

    • ab126598, at 1/50 dilution, staining Antithrombin III/ATIII in paraffin-embedded Human liver tissue by Immunohistochemistry.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Xu J  et al. Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells. Mol Med Rep 19:3658-3666 (2019). Read more (PubMed: 30896875) »
    • Cheng SX  et al. iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management. Neurotherapeutics 15:216-232 (2018). Read more (PubMed: 29247448) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Answer

    Lanes 1-4 in the western blot image on the datasheet https://www.abcam.com/antithrombin-iii-antibody-ep5372-ab126598.html are human plasma, ovary, testis, and fetal lung lysate, respectively. Thus, these were all tissue lysates rather than cell lysates.



    I did find ab180614 (https://www.abcam.com/antithrombin-iii-antibody-ab180614.html) and ab181689 (https://www.abcam.com/antithrombin-iii-antibody-n-terminal-ab181689.html), which were ran against the following cell lysates, which did not have protein secretion blocked. There was likely enough non-secreted antithrombin iii still in the cytoplasm of the cells to give positive bands.



    ab180614: U251, HepG2, PC3, Ho8910 cell lysates



    ab181689: SMMC, Jurkat, Raji cell lysates

    Read More

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