Recombinant Anti-Antithrombin III/ATIII antibody [EP5372] (ab126598)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5372] to Antithrombin III/ATIII
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Antithrombin III/ATIII antibody [EP5372]
See all Antithrombin III/ATIII primary antibodies -
Description
Rabbit monoclonal [EP5372] to Antithrombin III/ATIII -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human plasma, Mouse lung, and Rat lung lysate.
-
General notes
This product has switched from a hybridoma to recombinant production method on 31st May 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.26 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP5372 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
|
Notes |
---|
WB
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
Target
-
Function
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
Tissue specificity
Found in plasma. -
Involvement in disease
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
-
Database links
- Entrez Gene: 462 Human
- Entrez Gene: 11905 Mouse
- Entrez Gene: 304917 Rat
- Omim: 107300 Human
- SwissProt: P01008 Human
- SwissProt: P32261 Mouse
- SwissProt: Q5M7T5 Rat
- Unigene: 75599 Human
see all -
Alternative names
- ANT3_HUMAN antibody
- Antithrombin antibody
- Antithrombin III antibody
see all
Images
-
Anti-Antithrombin III/ATIII antibody [EP5372] (ab126598) at 1/10000 dilution + Human plasma lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with Human IgG at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
All lanes : Anti-Antithrombin III/ATIII antibody [EP5372] (ab126598) at 1/10000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1: 3 seconds; Lane 2: 80 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab126598 has been referenced in 10 publications.
- Wang C et al. Screening and identification of endometrial proteins as novel potential biomarkers for repeated implantation failure. PeerJ 9:e11009 (2021). PubMed: 33763303
- Jian X et al. Downregulation of microRNA-200c-3p alleviates the aggravation of venous thromboembolism by targeting serpin family C member 1. Bioengineered 12:11156-11168 (2021). PubMed: 34783290
- Bai J et al. iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy. Mol Med Rep 22:3607-3620 (2020). PubMed: 32901873
- Zhao L et al. Alterations in complement and coagulation pathways of human placentae subjected to in vitro fertilization and embryo transfer in the first trimester. Medicine (Baltimore) 98:e17031 (2019). PubMed: 31689742
- Xu J et al. Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells. Mol Med Rep 19:3658-3666 (2019). PubMed: 30896875
- Cheng SX et al. iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management. Neurotherapeutics 15:216-232 (2018). PubMed: 29247448
- Liu W et al. Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression. Mol Med Rep 17:2402-2414 (2018). PubMed: 29207079
- Gong J et al. Inhibition of FASN suppresses migration, invasion and growth in hepatoma carcinoma cells by deregulating the HIF-1a/IGFBP1 pathway. Int J Oncol 50:883-892 (2017). PubMed: 28197637
- Gillrie MR et al. Thrombin Cleavage of Plasmodium falciparum Erythrocyte Membrane Protein 1 Inhibits Cytoadherence. MBio 7:N/A (2016). PubMed: 27624125
- Wilson GJ et al. GCN2 is required to increase fibroblast growth factor 21 and maintain hepatic triglyceride homeostasis during asparaginase treatment. Am J Physiol Endocrinol Metab 308:E283-93 (2015). PubMed: 25491724