Product nameAnti-Antithrombin III/ATIII antibody [EPR5371]
See all Antithrombin III/ATIII primary antibodies
DescriptionRabbit monoclonal [EPR5371] to Antithrombin III/ATIII
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Antithrombin III/ATIII aa 1-100. The exact sequence is proprietary.
- Human plasma, ovary cancer and Human testis lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab124808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).|
FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Tissue specificityFound in plasma.
Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
Sequence similaritiesBelongs to the serpin family.
modificationsPhosphorylation sites are present in the extracelllular medium.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- ANT3_HUMAN antibody
- Antithrombin antibody
- Antithrombin III antibody
All lanes : Anti-Antithrombin III/ATIII antibody [EPR5371] (ab124808) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human ovary cancer lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
ab124808 has not yet been referenced specifically in any publications.