Recombinant
RabMAb

Recombinant Anti-Antithrombin III/ATIII antibody [EPR5371] (ab124808)

Overview

  • Product name

    Anti-Antithrombin III/ATIII antibody [EPR5371]
    See all Antithrombin III/ATIII primary antibodies
  • Description

    Rabbit monoclonal [EPR5371] to Antithrombin III/ATIII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Antithrombin III/ATIII aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human plasma, ovary cancer and Human testis lysates
  • General notes

     

     

    Previously labelled as Antithrombin III.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR5371
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
    • Tissue specificity

      Found in plasma.
    • Involvement in disease

      Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
    • Sequence similarities

      Belongs to the serpin family.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted > extracellular space.
    • Information by UniProt
    • Database links

    • Alternative names

      • ANT3_HUMAN antibody
      • Antithrombin antibody
      • Antithrombin III antibody
      • Antithrombin-III antibody
      • AntithrombinIII antibody
      • AT 3 antibody
      • AT III antibody
      • AT3 antibody
      • AT3D antibody
      • ATIII antibody
      • Heparin cofactor I antibody
      • MGC22579 antibody
      • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
      • Serine cysteine proteinase inhibitor clade C member 1 antibody
      • Serine proteinase inhibitor clade C member 1 antibody
      • Serpin C1 antibody
      • Serpin family C member 1 antibody
      • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
      • SERPINC1 antibody
      • THPH7 antibody
      see all

    Images

    • All lanes : Anti-Antithrombin III/ATIII antibody [EPR5371] (ab124808) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?

    References

    ab124808 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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