Key features and details
- Rabbit polyclonal to Antizyme inhibitor 1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig, Recombinant fragment
- Isotype: IgG
Product nameAnti-Antizyme inhibitor 1 antibody
DescriptionRabbit polyclonal to Antizyme inhibitor 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig, Recombinant fragment
Predicted to work with: Mouse, Rat, Orangutan
Recombinant fragment (His-tag) corresponding to Human Antizyme inhibitor 1 aa 5-290. (Expressed in E.coli).
IDDANYSVGLLDEGTNLGNVIDNYVYEHTLTGKNAFFVGDLGKIVKKHSQ WQNVVAQIKPFYTVKCNSAPAVLEILAALGTGFACSSKNEMALVQELGVP PENIIYISPCKQVSQIKYAAKVGVNILTCDNEIELKKIARNHPNAKVLLH IATEDNIGGEEGNMKFGTTLKNCRHLLECAKELDVQIIGVKFHVSSACKE SQVYVHALSDARCVFDMAGEIGFTMNMLDIGGGFTGTEFQLEEVNHVISP LLDIYFPEGSGVKIISEPGSYYVSSAFTLAVNIIAK
Database link: O14977
- WB: Recombinant human Antizyme inhbitor 1 protein; Pig liver lysate; HepG2 cell lysate. IHC-P: Human liver and stomach tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231342 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
FunctionRegulates cellular polyamine homeostasis (By similarity). Inhibits antizyme-dependent ornithine decarboxylase degradation by binding to antizyme.
Tissue specificityExpressed in liver.
Sequence similaritiesBelongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily.
- Information by UniProt
- Antizyme inhibitor 1 antibody
- AZI antibody
- Azin1 antibody
Anti-Antizyme inhibitor 1 antibody (ab231342) at 1 µg/ml + Recombinant human Antizyme inhibitor 1 protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 50 kDa
All lanes : Anti-Antizyme inhibitor 1 antibody (ab231342) at 1 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Paraffin-embedded human liver tissue stained for Antizyme inhibitor 1 using ab231342 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach tissue stained for Antizyme inhibitor 1 using ab231342 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231342 has not yet been referenced specifically in any publications.