Anti-Antizyme inhibitor 1 antibody (ab231342)
Key features and details
- Rabbit polyclonal to Antizyme inhibitor 1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Antizyme inhibitor 1 antibody -
Description
Rabbit polyclonal to Antizyme inhibitor 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig, Recombinant fragment
Predicted to work with: Mouse, Rat, Orangutan -
Immunogen
Recombinant fragment (His-tag) corresponding to Human Antizyme inhibitor 1 aa 1-300. (Expressed in E.coli).
Database link: O14977 -
Positive control
- WB: Recombinant human Antizyme inhbitor 1 protein; Pig liver lysate; HepG2 cell lysate. IHC-P: Human liver and stomach tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231342 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
Target
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Function
Regulates cellular polyamine homeostasis (By similarity). Inhibits antizyme-dependent ornithine decarboxylase degradation by binding to antizyme. -
Tissue specificity
Expressed in liver. -
Sequence similarities
Belongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily. - Information by UniProt
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Database links
- Entrez Gene: 51582 Human
- Entrez Gene: 54375 Mouse
- Entrez Gene: 100173300 Orangutan
- Entrez Gene: 58961 Rat
- Omim: 607909 Human
- SwissProt: O14977 Human
- SwissProt: O35484 Mouse
- SwissProt: Q5R7K3 Orangutan
see all -
Alternative names
- Antizyme inhibitor 1 antibody
- AZI antibody
- Azin1 antibody
see all
Images
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Anti-Antizyme inhibitor 1 antibody (ab231342) at 1 µg/ml + Recombinant human Antizyme inhibitor 1 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 50 kDa -
All lanes : Anti-Antizyme inhibitor 1 antibody (ab231342) at 1 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 50 kDa -
Paraffin-embedded human liver tissue stained for Antizyme inhibitor 1 using ab231342 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach tissue stained for Antizyme inhibitor 1 using ab231342 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231342 has not yet been referenced specifically in any publications.