RecombinantRabMAb

Recombinant Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)

Reviews (2)Q&A (3)
Western blot - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
  • Immunocytochemistry/ Immunofluorescence - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
  • Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7717] to ANTXR2/CMG-2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-ANTXR2/CMG-2 antibody [EPR7717]
    See all ANTXR2/CMG-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7717] to ANTXR2/CMG-2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ANTXR2/CMG-2 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, ECV304 and PC3 lysates; Human colon tissue; PC3 cells.

  • General notes

     

     

    Previously labelled as ANTXR2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Please note: ab129004 seems to be not suitable for the use in IHC-P with mouse samples.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Please note: ab129004 seems to be not suitable for the use in IHC-P with mouse samples.
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Necessary for cellular interactions with laminin and the extracellular matrix.

    • Tissue specificity

      Expressed in prostate, thymus, ovary, testis, pancreas, colon, heart, kidney, lung, liver, peripheral blood leukocytes, placenta, skeletal muscle, small intestine and spleen.

    • Involvement in disease

      Defects in ANTXR2 are the cause of infantile systemic hyalinosis (ISH) [MIM:236490]. This autosomal recessive syndrome is similar to JHF, but has an earlier onset and a more severe course. Symptoms appear at birth or within the first months of life, with painful, swollen joint contractures, osteopenia, osteoporosis and livid red hyperpigmentation over bony prominences. Patients develop multiple subcutaneous skin tumors and gingival hypertrophy. Hyaline deposits in multiple organs, recurrent infections and intractable diarrhea often lead to death within the first 2 years of life. Surviving children may suffer from severely reduced mobility due to joint contractures.
      Defects in ANTXR2 are the cause of juvenile hyaline fibromatosis (JHF) [MIM:228600]. JHF is an autosomal recessive syndrome that is similar to ISH but takes a milder course. It is characterized by hyaline deposition in the dermis, multiple subcutaneous skin tumors and gingival hypertrophy, followed by progressive joint contractions, osteopenia and osteoporosis that may lead to a severe limitation of mobility.

    • Sequence similarities

      Belongs to the ATR family.
      Contains 1 VWFA domain.

    • Domain

      Binding to PA seems to be effected through the VWA domain.

    • Cellular localization

      Secreted; Cell membrane. Expressed at the cell surface and Endoplasmic reticulum membrane. Expressed predominantly within the endoplasmic reticulum and not at the plasma membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 2310046B19Rik antibody
      • Anthrax toxin receptor 2 antibody
      • ANTR2_HUMAN antibody
      • Antxr2 antibody
      • AW561899 antibody
      • Capillary morphogenesis gene 2 protein antibody
      • Capillary morphogenesis protein 2 antibody
      • CMG 2 antibody
      • CMG-2 antibody
      • CMG2 antibody
      • FLJ31074 antibody
      • HFS antibody
      • ISH antibody
      • JHF antibody
      • MGC111533 antibody
      • MGC45856 antibody
      see all

    Images

    • Western blot - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
      Western blot - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
      All lanes : Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : ECV304 cell lysate
      Lane 3 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)

      ab129004, at 1/250 dilution, staining ANTXR2/CMG-2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
      Immunocytochemistry/ Immunofluorescence - Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)

      ab129004, at 1/250 dilution staining ANTXR2/CMG-2 in PC3 cells by Immunofluorescence.

    • Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
      Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)

    References (0)

    Publishing research using ab129004? Please let us know so that we can cite the reference in this datasheet.

    ab129004 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-ANTXR2 / CMG-2 antibody [EPR7717]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing (4-12% Tris-glycine)
    Loading amount
    20 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 30 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-ANTXR2 / CMG-2 antibody [EPR7717]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (liver, kidney)
    Permeabilization
    No
    Specification
    liver, kidney
    Blocking step
    HCl or none as blocking agent for 20 minute(s) · Concentration: 0.02% · Temperature: 20°C
    Fixative
    none
    Read More

    Nadine Stokar

    Verified customer

    Submitted Oct 29 2013

    Question

    I am planning on doing immunofluorescence with Anti-CMG2 antibody ab19004. I just wanted to get the complete protocol that was used for the immunofluorescence with the antibody on PC3 cells.

    Read More
    Answer

    Thank you for contacting us.

    The recommended ICC/IF protocol can be found here:

    http://www.epitomics.com/products/support/icc

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    Thanks for the information. Do you know anyone use these antibodies for IF and had publication?

    Read More
    Answer

    Thank you for contacting us.

    To our knowledge, neither of these antibodies have been used in any references yet. All of the known references for our products can be found on the Specific References tab of the online datasheet. Please let me know if you need any additional information or assistance.

    Read More

    Question

    I just order the products (ab129004 & ab21269) from your company. I will use them for immunofluorescence. I wonder if you have the step by step protocols for these two antibodies or if you know someone has done IF with these antibodies and how did they do?

    Read More
    Answer

    Thank you for contacting us.

    The attached ICC/IF protocol was used to validate ab129004 but would also be suitable for use with ab21269. The recommended dilutions for these antibodies are 1:250 - 500. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

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