Product nameANXA7 overexpression 293T lysate (whole cell)
DescriptionANXA7 overexpression lysate
ab94175 is a 293T cell transfected lysate in which Human Annexin VII has been transiently over-expressed using a pCMV-Annexin VII plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Tissue specificity: Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.
Our Abpromise guarantee covers the use of ab94175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94175 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Annexin-7/ANXA7 antibody (ab88539) at 1/500 dilution
Lane 1 :
ANXA7 overexpression 293T lysate (whole cell) (ab94175)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94175 has not yet been referenced specifically in any publications.