Anti-AP-2 complex subunit alpha-1 antibody (ab156806)

Overview

  • Product name
    Anti-AP-2 complex subunit alpha-1 antibody
    See all AP-2 complex subunit alpha-1 primary antibodies
  • Description
    Goat polyclonal to AP-2 complex subunit alpha-1
  • Host species
    Goat
  • Specificity
    ab156806 is expected to recognize reported isoform 1 (NP_055018.2) only.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 706-727 (internal sequence) (Cysteine residue).
    Sequence:

    ELEPPAPESPMALLADPAPAAD

  • Positive control
    • Human brain frontal cortex lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156806 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 108 kDa).

Target

  • Function
    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
  • Tissue specificity
    Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
  • Sequence similarities
    Belongs to the adaptor complexes large subunit family.
  • Cellular localization
    Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 100 kDa coated vesicle protein A antibody
    • Adapter-related protein complex 2 alpha-1 subunit antibody
    • adapter-related protein complex 2 subunit alpha-1 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor protein complex AP-2 subunit alpha-1 antibody
    • Adaptor protein complex AP2 alpha 1 subunit antibody
    • adaptor protein complex AP2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex AP 2 alpha 1 subunit antibody
    • Adaptor related protein complex AP2 alpha 1 subunit antibody
    • ADTAA antibody
    • Alpha A adaptin antibody
    • Alpha adaptin A antibody
    • Alpha-adaptin A antibody
    • alpha1 adaptin antibody
    • Alpha1-adaptin antibody
    • AP 2 alpha antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP-2 complex subunit alpha-1 antibody
    • AP2 complex subunit alpha 1 antibody
    • AP2-ALPHA antibody
    • Ap2a1 antibody
    • AP2A1_HUMAN antibody
    • CLAPA 1 antibody
    • CLAPA1 antibody
    • Clathrin adaptor complex AP2. alpha subunit antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha-A large chain antibody
    • Clathrin associated / assembly / adaptor protein large alpha 1 antibody
    • clathrin associated assembly adaptor protein large alpha 1 antibody
    • clathrin associated/assembly/adaptor protein, large, alpha 1 antibody
    • Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    see all

Images

  • Anti-AP-2 complex subunit alpha-1 antibody (ab156806) at 0.01 µg/ml + Human frontal cortex lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 108 kDa
    Observed band size: 110 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab156806 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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