Overview

  • Product name

    Anti-AP-2 complex subunit alpha-1 antibody
    See all AP-2 complex subunit alpha-1 primary antibodies
  • Description

    Goat polyclonal to AP-2 complex subunit alpha-1
  • Host species

    Goat
  • Specificity

    ab189995 is expected to recognize reported isoform 1 (NP_055018.2) only.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 706-727 (internal sequence) (Cysteine residue). NP_055018.2.
    Sequence:

    ELEPPAPESPMALLADPAPAAD


    Database link: O95782

  • Positive control

    • Human brain cortex tissue; HeLa cells, stably expressing a chimeric construct including the brain-specific splice variant of Mouse AP-2 complex subunit alpha-1; Mouse brain lysate and Human Frontal Cortex lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.1 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 108 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
  • Tissue specificity

    Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
  • Sequence similarities

    Belongs to the adaptor complexes large subunit family.
  • Cellular localization

    Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 100 kDa coated vesicle protein A antibody
    • Adapter-related protein complex 2 alpha-1 subunit antibody
    • adapter-related protein complex 2 subunit alpha-1 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor protein complex AP-2 subunit alpha-1 antibody
    • Adaptor protein complex AP2 alpha 1 subunit antibody
    • adaptor protein complex AP2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex AP 2 alpha 1 subunit antibody
    • Adaptor related protein complex AP2 alpha 1 subunit antibody
    • ADTAA antibody
    • Alpha A adaptin antibody
    • Alpha adaptin A antibody
    • Alpha-adaptin A antibody
    • alpha1 adaptin antibody
    • Alpha1-adaptin antibody
    • AP 2 alpha antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP-2 complex subunit alpha-1 antibody
    • AP2 complex subunit alpha 1 antibody
    • AP2-ALPHA antibody
    • Ap2a1 antibody
    • AP2A1_HUMAN antibody
    • CLAPA 1 antibody
    • CLAPA1 antibody
    • Clathrin adaptor complex AP2. alpha subunit antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha-A large chain antibody
    • Clathrin associated / assembly / adaptor protein large alpha 1 antibody
    • clathrin associated assembly adaptor protein large alpha 1 antibody
    • clathrin associated/assembly/adaptor protein, large, alpha 1 antibody
    • Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    see all

Images

  • Anti-AP-2 complex subunit alpha-1 antibody (ab189995) at 0.01 µg/ml + Human Frontal Cortex lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 108 kDa

  • Lanes 1-3 : total protein blot stained with Ponceau Red
    Lanes 4-6 : Anti-AP-2 complex subunit alpha-1 antibody (ab189995) at 0.1 µg/ml

    Lanes 1 & 4 : Mouse Liver lysate
    Lanes 2 & 5 : Mouse Brain lysate
    Lanes 3 & 6 : Mouse Kidney lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 108 kDa



    The left panel is stained with Ponceau red.

  • Immunofluorescent analysis of methanol-fixed HeLa cells stably expressing a chimeric construct including the brain-specific splice variant of Mouse AP-2 complex subunit alpha-1 labeling AP-2 complex subunit alpha-1 with ab189995 at 0.1 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling AP-2 complex subunit alpha-1 with ab189995 at 5µg/ml.

References

This product has been referenced in:

  • Nie X  et al. mTOR acts as a pivotal signaling hub for neural crest cells during craniofacial development. PLoS Genet 14:e1007491 (2018). Read more (PubMed: 29975682) »
  • Bagh MB  et al. Misrouting of v-ATPase subunit V0a1 dysregulates lysosomal acidification in a neurodegenerative lysosomal storage disease model. Nat Commun 8:14612 (2017). IP, ICC/IF ; Mouse, Human . Read more (PubMed: 28266544) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (cos7)
Permeabilization
Yes - 0.02% TritonX100
Specification
cos7
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde

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Verified customer

Submitted Dec 23 2015

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