Anti-AP-2 complex subunit alpha-1 antibody (ab3707)
Key features and details
- Goat polyclonal to AP-2 complex subunit alpha-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AP-2 complex subunit alpha-1 antibody
See all AP-2 complex subunit alpha-1 primary antibodies -
Description
Goat polyclonal to AP-2 complex subunit alpha-1 -
Host species
Goat -
Specificity
The immunogen sequence is found in both AP2A2 and AP2A1. This antibody recognises a band of just under 100kD in multiple human cell lines (see picture), the band can be blocked with the immunising peptide. -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 3-14.
Sequence:AVSKGDGMRGLAC
(Peptide available asab11698) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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All applications |
Mouse
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Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 104 , 108 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 104 , 108 kDa). |
Target
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Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. -
Tissue specificity
Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells. -
Sequence similarities
Belongs to the adaptor complexes large subunit family. -
Cellular localization
Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. - Information by UniProt
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Database links
- Entrez Gene: 160 Human
- Entrez Gene: 11771 Mouse
- Omim: 601026 Human
- SwissProt: O95782 Human
- SwissProt: P17426 Mouse
- Unigene: 467125 Human
- Unigene: 6877 Mouse
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Alternative names
- 100 kDa coated vesicle protein A antibody
- Adapter-related protein complex 2 alpha-1 subunit antibody
- adapter-related protein complex 2 subunit alpha-1 antibody
see all
Images
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Western blot using ab3707 at 1/500.
Lane 1: HeLa Nuclear Extract
Lane 2: HeLa Whole Cell Lysate
Lane 3: 293 Whole Cell Lysate
Lane 4: A431 Whole Cell Lysate
Lane 5: Jurkat Whole Cell Lysate
Lane 6: HeLa Nuclear Extract + blocking/immunising peptide
Lane 7: HeLa Whole Cell Lysate + blocking/immunising peptide
Lane 8: 293 Whole Cell Lysate + blocking/immunising peptide
Lane 9: A431 Whole Cell Lysate + blocking/immunising peptide
Lane 10: Jurkat Whole Cell Lysate + blocking/immunising peptide
AP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.
Secondary ab: Rabbit polyclonal to Goat IgG HRP
ab6741 (1/5000)
Exposure time: 1 minute.<
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab3707 has not yet been referenced specifically in any publications.