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Signal Transduction Protein Trafficking Vesicle Transport Adapters
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Anti-AP-2 complex subunit alpha-1 antibody (ab3707)

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Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab3707)

    Key features and details

    • Goat polyclonal to AP-2 complex subunit alpha-1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    View more associated products

    Overview

    • Product name

      Anti-AP-2 complex subunit alpha-1 antibody
      See all AP-2 complex subunit alpha-1 primary antibodies
    • Description

      Goat polyclonal to AP-2 complex subunit alpha-1
    • Host species

      Goat
    • Specificity

      The immunogen sequence is found in both AP2A2 and AP2A1. This antibody recognises a band of just under 100kD in multiple human cell lines (see picture), the band can be blocked with the immunising peptide.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 3-14.
      Sequence:

      AVSKGDGMRGLAC


      (Peptide available as ab11698)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Adapters

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3707 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Application Abreviews Notes
    WB
    1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 104 , 108 kDa).
    Notes
    WB
    1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 104 , 108 kDa).

    Target

    • Function

      Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
    • Tissue specificity

      Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
    • Sequence similarities

      Belongs to the adaptor complexes large subunit family.
    • Cellular localization

      Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
    • Target information above from: UniProt accession O95782 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 160 Human
      • Entrez Gene: 11771 Mouse
      • Omim: 601026 Human
      • SwissProt: O95782 Human
      • SwissProt: P17426 Mouse
      • Unigene: 467125 Human
      • Unigene: 6877 Mouse
      • Alternative names

        • 100 kDa coated vesicle protein A antibody
        • Adapter-related protein complex 2 alpha-1 subunit antibody
        • adapter-related protein complex 2 subunit alpha-1 antibody
        • Adaptin alpha A antibody
        • Adaptin alpha antibody
        • Adaptor protein complex AP 2 alpha 1 subunit antibody
        • adaptor protein complex AP 2 subunit alpha 1 antibody
        • Adaptor protein complex AP-2 subunit alpha-1 antibody
        • Adaptor protein complex AP2 alpha 1 subunit antibody
        • adaptor protein complex AP2 subunit alpha 1 antibody
        • Adaptor related protein complex 2 alpha 1 subunit antibody
        • Adaptor related protein complex AP 2 alpha 1 subunit antibody
        • Adaptor related protein complex AP2 alpha 1 subunit antibody
        • ADTAA antibody
        • Alpha A adaptin antibody
        • Alpha adaptin A antibody
        • Alpha-adaptin A antibody
        • alpha1 adaptin antibody
        • Alpha1-adaptin antibody
        • AP 2 alpha antibody
        • AP 2 complex subunit alpha 1 antibody
        • AP-2 complex subunit alpha-1 antibody
        • AP2 complex subunit alpha 1 antibody
        • AP2-ALPHA antibody
        • Ap2a1 antibody
        • AP2A1_HUMAN antibody
        • CLAPA 1 antibody
        • CLAPA1 antibody
        • Clathrin adaptor complex AP2. alpha subunit antibody
        • Clathrin assembly protein complex 2 alpha A large chain antibody
        • Clathrin assembly protein complex 2 alpha-A large chain antibody
        • Clathrin associated / assembly / adaptor protein large alpha 1 antibody
        • clathrin associated assembly adaptor protein large alpha 1 antibody
        • clathrin associated/assembly/adaptor protein, large, alpha 1 antibody
        • Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit antibody
        • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
        see all

      Images

      • Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab3707)
        Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab3707)

        Western blot using ab3707 at 1/500.

        Lane 1: HeLa Nuclear Extract
        Lane 2: HeLa Whole Cell Lysate
        Lane 3: 293 Whole Cell Lysate
        Lane 4: A431 Whole Cell Lysate
        Lane 5: Jurkat Whole Cell Lysate
        Lane 6: HeLa Nuclear Extract + blocking/immunising peptide
        Lane 7: HeLa Whole Cell Lysate + blocking/immunising peptide
        Lane 8: 293 Whole Cell Lysate + blocking/immunising peptide
        Lane 9: A431 Whole Cell Lysate + blocking/immunising peptide
        Lane 10: Jurkat Whole Cell Lysate + blocking/immunising peptide

        AP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.

        Secondary ab: Rabbit polyclonal to Goat IgG HRP
        ab6741 (1/5000)
        Exposure time: 1 minute.<

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab3707? Please let us know so that we can cite the reference in this datasheet.

      ab3707 has not yet been referenced specifically in any publications.

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