• Product name
    Anti-AP-2 complex subunit alpha-1 antibody
    See all AP-2 complex subunit alpha-1 primary antibodies
  • Description
    Goat polyclonal to AP-2 complex subunit alpha-1
  • Host species
  • Specificity
    The immunogen sequence is found in both AP2A2 and AP2A1. This antibody recognises a band of just under 100kD in multiple human cell lines (see picture), the band can be blocked with the immunising peptide.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 3-14.


    (Peptide available as ab11698)


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 104 , 108 kDa).


  • Function
    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
  • Tissue specificity
    Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
  • Sequence similarities
    Belongs to the adaptor complexes large subunit family.
  • Cellular localization
    Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 100 kDa coated vesicle protein A antibody
    • Adapter-related protein complex 2 alpha-1 subunit antibody
    • adapter-related protein complex 2 subunit alpha-1 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor protein complex AP-2 subunit alpha-1 antibody
    • Adaptor protein complex AP2 alpha 1 subunit antibody
    • adaptor protein complex AP2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex AP 2 alpha 1 subunit antibody
    • Adaptor related protein complex AP2 alpha 1 subunit antibody
    • ADTAA antibody
    • Alpha A adaptin antibody
    • Alpha adaptin A antibody
    • Alpha-adaptin A antibody
    • alpha1 adaptin antibody
    • Alpha1-adaptin antibody
    • AP 2 alpha antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP-2 complex subunit alpha-1 antibody
    • AP2 complex subunit alpha 1 antibody
    • AP2-ALPHA antibody
    • Ap2a1 antibody
    • AP2A1_HUMAN antibody
    • CLAPA 1 antibody
    • CLAPA1 antibody
    • Clathrin adaptor complex AP2. alpha subunit antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha-A large chain antibody
    • Clathrin associated / assembly / adaptor protein large alpha 1 antibody
    • clathrin associated assembly adaptor protein large alpha 1 antibody
    • clathrin associated/assembly/adaptor protein, large, alpha 1 antibody
    • Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    see all


  • Western blot using ab3707 at 1/500.

    Lane 1: HeLa Nuclear Extract
    Lane 2: HeLa Whole Cell Lysate
    Lane 3: 293 Whole Cell Lysate
    Lane 4: A431 Whole Cell Lysate
    Lane 5: Jurkat Whole Cell Lysate
    Lane 6: HeLa Nuclear Extract + blocking/immunising peptide
    Lane 7: HeLa Whole Cell Lysate + blocking/immunising peptide
    Lane 8: 293 Whole Cell Lysate + blocking/immunising peptide
    Lane 9: A431 Whole Cell Lysate + blocking/immunising peptide
    Lane 10: Jurkat Whole Cell Lysate + blocking/immunising peptide

    AP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.

    Secondary ab: Rabbit polyclonal to Goat IgG HRP
    ab6741 (1/5000)
    Exposure time: 1 minute.<


ab3707 has not yet been referenced specifically in any publications.

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