Recombinant Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] (ab170955)


  • Product name

    Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)]
    See all AP-2 complex subunit alpha-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7573(2)(B)] to AP-2 complex subunit alpha-1
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human AP-2 complex subunit alpha-1 aa 950 to the C-terminus. The exact sequence is proprietary.
    Database link: O95782

  • Positive control

    • HeLa, A431, 293T and HepG2 whole cell lysate (ab7900).
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab170955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
    • Tissue specificity

      Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
    • Sequence similarities

      Belongs to the adaptor complexes large subunit family.
    • Cellular localization

      Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
    • Information by UniProt
    • Database links

    • Alternative names

      • 100 kDa coated vesicle protein A antibody
      • Adapter-related protein complex 2 alpha-1 subunit antibody
      • adapter-related protein complex 2 subunit alpha-1 antibody
      • Adaptin alpha A antibody
      • Adaptin alpha antibody
      • Adaptor protein complex AP 2 alpha 1 subunit antibody
      • adaptor protein complex AP 2 subunit alpha 1 antibody
      • Adaptor protein complex AP-2 subunit alpha-1 antibody
      • Adaptor protein complex AP2 alpha 1 subunit antibody
      • adaptor protein complex AP2 subunit alpha 1 antibody
      • Adaptor related protein complex 2 alpha 1 subunit antibody
      • Adaptor related protein complex AP 2 alpha 1 subunit antibody
      • Adaptor related protein complex AP2 alpha 1 subunit antibody
      • ADTAA antibody
      • Alpha A adaptin antibody
      • Alpha adaptin A antibody
      • Alpha-adaptin A antibody
      • alpha1 adaptin antibody
      • Alpha1-adaptin antibody
      • AP 2 alpha antibody
      • AP 2 complex subunit alpha 1 antibody
      • AP-2 complex subunit alpha-1 antibody
      • AP2 complex subunit alpha 1 antibody
      • AP2-ALPHA antibody
      • Ap2a1 antibody
      • AP2A1_HUMAN antibody
      • CLAPA 1 antibody
      • CLAPA1 antibody
      • Clathrin adaptor complex AP2. alpha subunit antibody
      • Clathrin assembly protein complex 2 alpha A large chain antibody
      • Clathrin assembly protein complex 2 alpha-A large chain antibody
      • Clathrin associated / assembly / adaptor protein large alpha 1 antibody
      • clathrin associated assembly adaptor protein large alpha 1 antibody
      • clathrin associated/assembly/adaptor protein, large, alpha 1 antibody
      • Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit antibody
      • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
      see all


    • All lanes : Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] (ab170955) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 108 kDa


    ab170955 has not yet been referenced specifically in any publications.

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