Overview

  • Product name
    Anti-AP-A antibody - C-terminal
    See all AP-A primary antibodies
  • Description
    Rabbit polyclonal to AP-A - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide within Human AP-A aa 906-938 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q07075

  • Positive control
    • NCI-H460 cells and cell lysates; Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 109 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Anti-AP-A antibody - C-terminal (ab170722) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size: 109 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling AP-A with ab170722 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometric analysis of NCI-H460 cells labeling AP-A with ab170722 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab170722 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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