Recombinant
RabMAb

Recombinant Anti-AP-A antibody [EPR9411(2)] (ab155991)

Overview

  • Product name

    Anti-AP-A antibody [EPR9411(2)]
    See all AP-A primary antibodies
  • Description

    Rabbit monoclonal [EPR9411(2)] to AP-A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AP-A. The exact sequence is proprietary.

  • Positive control

    • Human fetal lung and fetal kidney lysates; Human kidney and placenta tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 109 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
    • Tissue specificity

      Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
    • Sequence similarities

      Belongs to the peptidase M1 family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Aminopeptidase A antibody
      • AMPE_HUMAN antibody
      • AP-A antibody
      • APA antibody
      • bp-1 antibody
      • bp1 antibody
      • CD249 antibody
      • Differentiation antigen gp160 antibody
      • EAP antibody
      • Enpep antibody
      • Glutamyl aminopeptidase (aminopeptidase A) antibody
      • Glutamyl aminopeptidase antibody
      • Gp160 antibody
      • Ly51 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AP-A with ab155991 at 1/100 dilution.

    • All lanes : Anti-AP-A antibody [EPR9411(2)] (ab155991) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 109 kDa

    • ab155991 showing +ve staining in Human vascular endothelium.
    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AP-A with ab155991 at 1/100 dilution.

    • ab155991 showing -ve staining in Human normal prostate.
    • ab155991 showing -ve staining in Human ovarian carcinoma.
    • ab155991 showing -ve staining in Human normal tonsil.

    References

    ab155991 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
    Permeabilization
    Yes - 0.05% tween 20 in the washing buffer
    Specification
    mouse kidney
    Blocking step
    commercial - Invitrogen's CAS-Block as blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 21 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (adult and fetal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
    Permeabilization
    Yes - 0.05% tween 20 in the washing buffer
    Specification
    adult and fetal kidney
    Blocking step
    commercial - Invitrogen's CAS-Block as blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 03 2015

    This product is known to not work in this application or species.
    Application
    Flow Cytometry
    Sample
    Human Cell (hESC H9)
    Permeabilization
    No
    Gating Strategy
    Doublet discrimination gating/live-dead discrimination (PI)/GFP-expressing cells
    Specification
    hESC H9
    Preparation
    Cell harvesting/tissue preparation method: mild trypsinisation/non-enzymatic dissociation
    Sample buffer: FACS staining buffer
    Fixation
    live non-fixed cells

    Dr. Dmitry Shaposhnikov

    Verified customer

    Submitted Nov 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up