Recombinant Anti-AP-A antibody [EPR9411(2)] (ab155991)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)](https://www.abcam.com/ps/products/155/ab155991/Images/ab155991-163314-anti-ap-a-antibody-epr94112-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9411(2)] to AP-A
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-AP-A antibody [EPR9411(2)]
See all AP-A primary antibodies -
Description
Rabbit monoclonal [EPR9411(2)] to AP-A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AP-A. The exact sequence is proprietary.
-
Positive control
- Human fetal lung and fetal kidney lysates; Human kidney and placenta tissue.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9411(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 109 kDa.
|
|
| IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 109 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. -
Tissue specificity
Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues. -
Sequence similarities
Belongs to the peptidase M1 family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2028 Human
- Omim: 138297 Human
- SwissProt: Q07075 Human
- Unigene: 435765 Human
-
Alternative names
- Aminopeptidase A antibody
- AMPE_HUMAN antibody
- AP-A antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AP-A with ab155991 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-AP-A antibody [EPR9411(2)] (ab155991)](https://www.abcam.com/ps/products/155/ab155991/Images/ab155991-163315-anti-ap-a-antibody-epr94112-western-blot.jpg)
Western blot - Anti-AP-A antibody [EPR9411(2)] (ab155991)All lanes : Anti-AP-A antibody [EPR9411(2)] (ab155991) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 109 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)](https://www.abcam.com/ps/products/155/ab155991/Images/ab155991-168833-anti-ap-a-antibody-epr94112-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)ab155991 showing +ve staining in Human vascular endothelium.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)](https://www.abcam.com/ps/products/155/ab155991/Images/ab155991-163313-anti-ap-a-antibody-epr94112-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AP-A with ab155991 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)](https://www.abcam.com/ps/products/155/ab155991/Images/ab155991-168836-anti-ap-a-antibody-epr94112-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)ab155991 showing -ve staining in Human normal prostate.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)](https://www.abcam.com/ps/products/155/ab155991/Images/ab155991-168835-anti-ap-a-antibody-epr94112-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)ab155991 showing -ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)](https://www.abcam.com/ps/products/155/ab155991/Images/ab155991-168834-anti-ap-a-antibody-epr94112-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)ab155991 showing -ve staining in Human normal tonsil.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-AP-A antibody [EPR9411(2)] (ab155991)](https://www.abcam.com/ps/products/155/ab155991/Images/ab155991-8-benefits-of-recombinant-antibodies.png)
Anti-AP-A antibody [EPR9411(2)] (ab155991)
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab155991 has not yet been referenced specifically in any publications.
