Name: Anti-AP-A antibody [EPR9411(2)]
Brand: RabMAb
SKU: ab155991
Rating: 3 (3 reviews)

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Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9411(2)] to AP-A
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-AP-A antibody [EPR9411(2)]
See all AP-A primary antibodies
Description

Rabbit monoclonal [EPR9411(2)] to AP-A
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human AP-A. The exact sequence is proprietary.
Positive control
- Human fetal lung and fetal kidney lysates; Human kidney and placenta tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9411(2)
Isotype

IgG
Research areas

Alternative Versions
- Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (ab249235)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Our Abpromise guarantee covers the use of ab155991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 109 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC or IP.

Function

Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
Tissue specificity

Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
Sequence similarities

Belongs to the peptidase M1 family.
Cellular localization

Membrane.
Target information above from: UniProt accession Q07075 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2028 Human
- Omim: 138297 Human
- SwissProt: Q07075 Human
- Unigene: 435765 Human
Alternative names
- Aminopeptidase A antibody
- AMPE_HUMAN antibody
- AP-A antibody
- APA antibody
- bp-1 antibody
- bp1 antibody
- CD249 antibody
- Differentiation antigen gp160 antibody
- EAP antibody
- Enpep antibody
- Glutamyl aminopeptidase (aminopeptidase A) antibody
- Glutamyl aminopeptidase antibody
- Gp160 antibody
- Ly51 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AP-A with ab155991 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-AP-A antibody [EPR9411(2)] (ab155991)

All lanes : Anti-AP-A antibody [EPR9411(2)] (ab155991) at 1/1000 dilution

Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 109 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

ab155991 showing +ve staining in Human vascular endothelium.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AP-A with ab155991 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

ab155991 showing -ve staining in Human normal prostate.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

ab155991 showing -ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

ab155991 showing -ve staining in Human normal tonsil.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet

Publishing research using ab155991? Please let us know so that we can cite the reference in this datasheet.

ab155991 has not yet been referenced specifically in any publications.

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (mouse kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)

Permeabilization

Yes - 0.05% tween 20 in the washing buffer

Specification

mouse kidney

Blocking step

commercial - Invitrogen's CAS-Block as blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 21 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (adult and fetal kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)

Permeabilization

Yes - 0.05% tween 20 in the washing buffer

Specification

adult and fetal kidney

Blocking step

commercial - Invitrogen's CAS-Block as blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 03 2015

Application

Flow Cytometry

Sample

Human Cell (hESC H9)

Permeabilization

Gating Strategy

Doublet discrimination gating/live-dead discrimination (PI)/GFP-expressing cells

Specification

hESC H9

Preparation

Cell harvesting/tissue preparation method: mild trypsinisation/non-enzymatic dissociation
Sample buffer: FACS staining buffer

Fixation

live non-fixed cells

Dr. Dmitry Shaposhnikov

Verified customer

Submitted Nov 05 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-AP-A antibody [EPR9411(2)] (ab155991)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Applications

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AP-A antibody [EPR9411(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AP-A antibody [EPR9411(2)]

Flow Cytometry abreview for Anti-AP-A antibody [EPR9411(2)]