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  1. Link

    ap-a-antibody-epr94112-ab155991.pdf

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Immunology Adaptive Immunity B Cells CD
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RecombinantRabMAb

Recombinant Anti-AP-A antibody [EPR9411(2)] (ab155991)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
  • Western blot - Anti-AP-A antibody [EPR9411(2)] (ab155991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
  • Anti-AP-A antibody [EPR9411(2)] (ab155991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9411(2)] to AP-A
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-AP-A antibody [EPR9411(2)]
    See all AP-A primary antibodies
  • Description

    Rabbit monoclonal [EPR9411(2)] to AP-A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AP-A. The exact sequence is proprietary.

  • Positive control

    • Human fetal lung and fetal kidney lysates; Human kidney and placenta tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9411(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Hormones
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Associated products

  • Alternative Versions

    • Anti-AP-A antibody [EPR9411(2)] - BSA and Azide free (ab249235)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 109 kDa.
IHC-P (2)
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 109 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
  • Tissue specificity

    Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
  • Sequence similarities

    Belongs to the peptidase M1 family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q07075 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2028 Human
    • Omim: 138297 Human
    • SwissProt: Q07075 Human
    • Unigene: 435765 Human
    • Alternative names

      • Aminopeptidase A antibody
      • AMPE_HUMAN antibody
      • AP-A antibody
      • APA antibody
      • bp-1 antibody
      • bp1 antibody
      • CD249 antibody
      • Differentiation antigen gp160 antibody
      • EAP antibody
      • Enpep antibody
      • Glutamyl aminopeptidase (aminopeptidase A) antibody
      • Glutamyl aminopeptidase antibody
      • Gp160 antibody
      • Ly51 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AP-A with ab155991 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-AP-A antibody [EPR9411(2)] (ab155991)
      Western blot - Anti-AP-A antibody [EPR9411(2)] (ab155991)
      All lanes : Anti-AP-A antibody [EPR9411(2)] (ab155991) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 109 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

      ab155991 showing +ve staining in Human vascular endothelium.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AP-A with ab155991 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

      ab155991 showing -ve staining in Human normal prostate.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

      ab155991 showing -ve staining in Human ovarian carcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9411(2)] (ab155991)

      ab155991 showing -ve staining in Human normal tonsil.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-AP-A antibody [EPR9411(2)] (ab155991)
      Anti-AP-A antibody [EPR9411(2)] (ab155991)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab155991? Please let us know so that we can cite the reference in this datasheet.

    ab155991 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AP-A antibody [EPR9411(2)]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
    Permeabilization
    Yes - 0.05% tween 20 in the washing buffer
    Specification
    mouse kidney
    Blocking step
    commercial - Invitrogen's CAS-Block as blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 21 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AP-A antibody [EPR9411(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (adult and fetal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
    Permeabilization
    Yes - 0.05% tween 20 in the washing buffer
    Specification
    adult and fetal kidney
    Blocking step
    commercial - Invitrogen's CAS-Block as blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 03 2015

    Flow Cytometry abreview for Anti-AP-A antibody [EPR9411(2)]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (hESC H9)
    Permeabilization
    No
    Gating Strategy
    Doublet discrimination gating/live-dead discrimination (PI)/GFP-expressing cells
    Specification
    hESC H9
    Preparation
    Cell harvesting/tissue preparation method: mild trypsinisation/non-enzymatic dissociation
    Sample buffer: FACS staining buffer
    Fixation
    live non-fixed cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dmitry Shaposhnikov

    Verified customer

    Submitted Nov 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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