Key features and details
- Rabbit polyclonal to AP-B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AP-B antibody
See all AP-B primary antibodies
DescriptionRabbit polyclonal to AP-B
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human gallbladder tissue; RT-4 and U-251 MG cell lysates; Human liver and tonsil lysates; U-2 OS cells.
Previously labelled as RNPEP.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 73 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionExopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4).
Sequence similaritiesBelongs to the peptidase M1 family.
- Information by UniProt
- Aminopeptidase B antibody
- AMPB_HUMAN antibody
- Ap B antibody
All lanes : Anti-AP-B antibody (ab220088) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 73 kDa
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue labeling AP-B with ab220088 at 1/500 dilution.
Immunofluorecent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling AP-B with ab220088 at 4 µg/ml (green).
ab220088 has not yet been referenced specifically in any publications.