Overview

  • Product name

  • Description

    Rabbit polyclonal to AP1+2 beta
  • Host species

    Rabbit
  • Specificity

    Based on sequence analysis, this antibody will bind the beta subunit of both the AP-1 and AP-2 complexes.
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    KKGEIFELKAELNSDKKEK

    , corresponding to N terminal amino acids 10-29 of AP1 and 2 (beta).

  • Positive control

    • Rat brain lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Purification notes

    0.2ml filtered lyophilized serum.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-Fr
IHC-P
  • Application notes
    IHC-Fr: 1/500 - 1/5000. depending on tissue and
    detection method.
    IHC-P: 1/500 - 1/5000. depending on tissue and
    detection method.
    WB: 1/2000 - 1/10000. depending on detection method. Predicted molecular weight: 100 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References

    This product has been referenced in:

    • Wang L  et al. Prazosin protects myocardial cells against anoxia-reoxygenation injury via the extracellular signal-regulated kinase signaling pathway. Mol Med Rep 17:2145-2152 (2018). Read more (PubMed: 29207167) »
    • Zhu G  et al. miR-371b-5p inhibits endothelial cell apoptosis in monocrotaline-induced pulmonary arterial hypertension via PTEN/PI3K/Akt signaling pathways. Mol Med Rep 18:5489-5501 (2018). Read more (PubMed: 30387816) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% Triton x 100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 08 2019

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (kidney)
    Specification
    kidney
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3%

    Dr. Immaculada Herrero-Fresneda

    Verified customer

    Submitted Jan 05 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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