Key features and details
- Rabbit polyclonal to AP1+2 beta
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AP1+2 beta antibody
DescriptionRabbit polyclonal to AP1+2 beta
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: RT-4 and U-251 MG cell lysate. Human liver and tonsil tissue lysate. ICC/IF: HEK 293 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 105 kDa.|
FunctionSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the adaptor complexes large subunit family.
Cellular localizationGolgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
- Information by UniProt
- Adaptin, beta 2 (beta) antibody
- Adaptor protein complex AP-1 subunit beta-1 antibody
- Adaptor related protein complex 1, beta 1 subunit antibody
HEK 293 cells stained for AP1+2 beta (green) using ab221562 (4 µg/ml) in ICC/IF.
All lanes : Anti-AP1+2 beta antibody (ab221562) at 1/1000 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 105 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab221562 has not yet been referenced specifically in any publications.