Anti-AP1M2 antibody (ab220678)
Key features and details
- Rabbit polyclonal to AP1M2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AP1M2 antibody -
Description
Rabbit polyclonal to AP1M2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human AP1M2 aa 1-100.
Database link: Q9Y6Q5 -
Positive control
- Caco2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. -
Sequence similarities
Belongs to the adaptor complexes medium subunit family.
Contains 1 MHD (mu homology) domain. -
Post-translational
modificationsPhosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals. -
Cellular localization
Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 515766 Cow
- Entrez Gene: 10053 Human
- Entrez Gene: 11768 Mouse
- Omim: 607309 Human
- SwissProt: Q3SYW1 Cow
- SwissProt: Q9Y6Q5 Human
- SwissProt: Q9WVP1 Mouse
- Unigene: 18894 Human
see all -
Alternative names
- Adaptor protein complex AP-1 mu-2 subunit antibody
- Adaptor protein complex AP1 mu2 subunit antibody
- Adaptor related protein complex 1 mu2 subunit antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab220678 has not yet been referenced specifically in any publications.