Key features and details
- Rabbit polyclonal to AP1S1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-AP1S1 antibody
DescriptionRabbit polyclonal to AP1S1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
- HeLa, 293T, Jurkat, NIH 3T3, TCMK1 whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab217055 was affinity purified using an epitope specific to AP1S1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab217055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 19 kDa.|
FunctionSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the adaptor complexes small subunit family.
Cellular localizationGolgi apparatus. Cytoplasmic vesicle membrane. Membrane > clathrin-coated pit. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
- Information by UniProt
- Adapter related protein complex 1 sigma 1A subunit antibody
- Adapter-related protein complex 1 sigma-1A subunit antibody
- Adaptor protein complex AP-1 sigma-1A subunit antibody
All lanes : Anti-AP1S1 antibody (ab217055) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK1 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 10 seconds
cells prepared using NETN lysis buffer
Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling AP1S1 using ab217055 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated AP1S1, ab217055 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab217055 has not yet been referenced specifically in any publications.