Overview

  • Product name

    Anti-AP2 alpha + beta antibody [A6/2/2]
  • Description

    Mouse monoclonal [A6/2/2] to AP2 alpha + beta
  • Host species

    Mouse
  • Specificity

    Reacts with both AP-2 alpha and beta forms. As the antibody also recognises AP2 beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta.
  • Tested applications

    Suitable for: Flow Cyt, WB, ELISA, IHC-P, RIA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    C-terminal peptide of AP-2 alpha (PNSHTDNNAKSSDKEEKHRK).

  • Positive control

    • Raji cells or breast carcinoma

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    A6/2/2
  • Myeloma

    NS0
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Relevance

    The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer. AP2 alpha is the only AP2 protein required for early morphogenesis of the lens vesicle. AP2 beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Activating enhancer binding protein 2 alpha antibody
    • Activating enhancer binding protein 2 beta antibody
    • Activating enhancer-binding protein 2-alpha antibody
    • Activator protein 2 antibody
    • AP 2 antibody
    • AP 2alpha antibody
    • AP 2B antibody
    • AP-2 antibody
    • AP-2 transcription factor antibody
    • AP2 B antibody
    • AP2-alpha antibody
    • AP2-beta antibody
    • AP2TF antibody
    • BOFS antibody
    • TFAP2 antibody
    • TFAP2A antibody
    • TFAP2B antibody
    • Transcription factor AP2 alpha antibody
    • Transcription factor AP2 beta antibody
    see all

Images

  • ICC/IF image of ab61 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Gee JM  et al. Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in invasive breast cancer. J Pathol 189:514-20 (1999). Read more (PubMed: 10629551) »
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - nuclear (Hela)
Loading amount
30 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing

Abcam user community

Verified customer

Submitted Jul 30 2008

Question
Answer

Thank you for your enquiry. Raji cells or breast carcinomas should be a suitable positive control. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Question
Answer

The antibody A6/2/2 was raised against the peptide PNSHTDNNAKSSDKEEKHRK which is the C-terminus of AP-2alpha. As the antibody also recognises AP 2beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta. The antibody is described in: Gee, J.M.W., Robertson, J.F.R., Ellis,I.O., Nicholson,R.I. and Hurst,H.C.(1999). Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in breast cancer. J. Pathol. 189, 514-520

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Question
Answer

We always recommend that conditions for WB should be optimised by the end user. We suggest starting with a 1:1000 dilution.

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Answer

This antibody recognises the transcription factor AP-2 and thus not the adaptor protein (AP-2).

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Question
Answer

We don't have any information on this antibody being used in EMSA, but as the immunogen was a C-terminal peptide chances are quite high it will work.

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Answer

This antibody works in immunohistochemistry with paraffin embedded sections (fixative formal saline with calcium, 10 min microwave treatment required). However it has not been tested for whole mount IHC as you describe.

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