Key features and details
- Mouse monoclonal [A6/2/2] to AP2 alpha + beta
- Suitable for: Flow Cyt, WB, ELISA, IHC-P, RIA, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-AP2 alpha + beta antibody [A6/2/2]
DescriptionMouse monoclonal [A6/2/2] to AP2 alpha + beta
SpecificityReacts with both AP-2 alpha and beta forms. As the antibody also recognises AP2 beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta.
Tested applicationsSuitable for: Flow Cyt, WB, ELISA, IHC-P, RIA, ICC/IFmore details
Species reactivityReacts with: Human
C-terminal peptide of AP-2 alpha (PNSHTDNNAKSSDKEEKHRK).
- Raji cells or breast carcinoma
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityProtein A purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab61 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|RIA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
RelevanceThe AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer. AP2 alpha is the only AP2 protein required for early morphogenesis of the lens vesicle. AP2 beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia
- Activating enhancer binding protein 2 alpha antibody
- Activating enhancer binding protein 2 beta antibody
- Activating enhancer-binding protein 2-alpha antibody
ICC/IF image of ab61 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab61 has been referenced in 1 publication.
- Gee JM et al. Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in invasive breast cancer. J Pathol 189:514-20 (1999). PubMed: 10629551