Key features and details
- Rabbit polyclonal to AP2 beta
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AP2 beta antibody
See all AP2 beta primary antibodies
DescriptionRabbit polyclonal to AP2 beta
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Zebrafish
- Transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab50906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
|WB||Use a concentration of 2.5 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionSequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
Involvement in diseaseDefects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
Sequence similaritiesBelongs to the AP-2 family.
modificationsSumoylated on Lys-21; which inhibits transcriptional activity.
- Information by UniProt
- Activating enhancer binding protein 2 beta antibody
- Activating enhancer-binding protein 2-beta antibody
- AP 2B antibody
Anti-AP2 beta antibody (ab50906) at 1/2.5 dilution + Transfected 293T cell lysate
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab50906 has been referenced in 1 publication.
- Williams MJ et al. Obesity-linked homologues TfAP-2 and Twz establish meal frequency in Drosophila melanogaster. PLoS Genet 10:e1004499 (2014). PubMed: 25187989