The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
Use at an assay dependent concentration.
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
Involvement in disease
Defects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
Belongs to the AP-2 family.
Sumoylated on Lys-21; which inhibits transcriptional activity.
Activating enhancer binding protein 2 beta antibody
Activating enhancer-binding protein 2-beta antibody
AP 2B antibody
AP2 B antibody
TFAP 2B antibody
Transcription factor AP 2 beta antibody
Transcription factor AP-2-beta antibody
Transcription factor AP2 beta antibody
Immunohistochemistry (Frozen sections) - Anti-AP2 beta antibody [AP2b 1F3/8] (ab18113)This image is courtesy of an Abreview submitted by Dr Sophie Pezet
ab18113 at 1/300 staining perfusion fixed rat brain tissue sections by IHC-Fr. Adult rat was perfused intracardially with paraformaldehyde 4% + 15% of a saturated solution of picric acid in PB 0.2M. The brain was post-fixed in the same fixative for 24 hours. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1/300) and revealed with secondary antibody conjugated with Alexa Fluor ® 488 (1/2000). The staining obtained is restricted to the cytoplasm and consists of a small and thin punctate staining (white arrows). The pictures show the staining obtained in cortical neurons, with the X20 (top picture) and X40 (bottom picture) objective.
Bökenkamp R et al. Dlx1 and Rgs5 in the ductus arteriosus: vessel-specific genes identified by transcriptional profiling of laser-capture microdissected endothelial and smooth muscle cells. PLoS One9:e86892 (2014).
Read more (PubMed: 24489801) »
Ebauer M et al. Comparative expression profiling identifies an in vivo target gene signature with TFAP2B as a mediator of the survival function of PAX3/FKHR. Oncogene26:7267-81 (2007).
Read more (PubMed: 17525748) »