The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000. Predicted molecular weight: 49 kDa. Block with BSA only (not milk).
1/100 - 1/250.
1/50 - 1/500.
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
Belongs to the AP-2 family.
The WW-binding motif mediates interaction with WWOX.
Sumoylated on Lys-10; which inhibits transcriptional activity.