Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab110635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/100 - 1/1000. Predicted molecular weight: 49 kDa. Block with BSA only (not milk).
IP 1/100 - 1/250.
ICC/IF 1/50 - 1/500.

Target

  • Function

    Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
  • Sequence similarities

    Belongs to the AP-2 family.
  • Domain

    The WW-binding motif mediates interaction with WWOX.
  • Post-translational
    modifications

    Sumoylated on Lys-10; which inhibits transcriptional activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activating enhancer binding protein 2 gamma antibody
    • Activating enhancer-binding protein 2 gamma antibody
    • AP2-gamma antibody
    • AP2C_HUMAN antibody
    • AP2g antibody
    • AP2gamma antibody
    • ERF 1 antibody
    • ERF1 antibody
    • Estrogen receptor factor 1 antibody
    • hAP 2g antibody
    • hAP2g antibody
    • TFAP 2C antibody
    • TFAP 2G antibody
    • Tfap2c antibody
    • TFAP2G antibody
    • Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
    • Transcription factor AP 2 gamma antibody
    • Transcription factor AP-2 gamma antibody
    • Transcription factor ERF 1 antibody
    • Transcription factor ERF-1 antibody
    • Transcription factor ERF1 antibody
    see all

Images

  • Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in Hela Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • All lanes : Anti-AP2 gamma/TFAP2C antibody [3B5] (ab110635) at 1/50 dilution

    Lane 1 : PC-3 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Mouse placenta cell lysate
    Lane 4 : RAW 264.7 cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG (H+L), HRP conjugate at 1/4000 dilution

    Predicted band size: 49 kDa

  • Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with ab110635 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in U251 Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in MCF-7 Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

References

ab110635 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (Human dermal fibroblasts)
Permeabilization
Yes - BD Perm/Wash buffer 1X
Gating Strategy
Live cells
Specification
Human dermal fibroblasts
Preparation
Cell harvesting/tissue preparation method: Trypsin
Sample buffer: BD Perm/Wash buffer 1X
Fixation
BD Cytofix/Cytoperm solution

Abcam user community

Verified customer

Submitted Jan 06 2017

Answer

DISCOUNT CODE: 40365
Expiration date: 21/11/2012

I am very pleased to hear you would like to accept our offer and testab110635 in ChIP. This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN before the expiration date. To redeem this offer, please submit an Abreview forChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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