Name: Anti-AP2 gamma/TFAP2C antibody [EP2692Y]
Brand: RabMAb
SKU: ab76007
Rating: 5 (1 reviews)

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Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2692Y] to AP2 gamma/TFAP2C
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-AP2 gamma/TFAP2C antibody [EP2692Y]
See all AP2 gamma/TFAP2C primary antibodies
Description

Rabbit monoclonal [EP2692Y] to AP2 gamma/TFAP2C
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human AP2 gamma/TFAP2C aa 100-200. The exact sequence is proprietary.
Positive control
- Purchase matching WB positive control:Recombinant Human AP2 gamma/TFAP2C protein
- WB: MCF7 and SKBR3 cell lysates. IHC-P: Human cervical carcinoma, endometrial carcinoma and prostatic carcinoma tissues. ICC/IF: MCF7 cells Flow Cyt: MCF7 cells
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as AP2 gamma

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2692Y
Isotype

IgG
Research areas

Alternative Versions
- Anti-AP2 gamma/TFAP2C antibody [EP2692Y] - BSA and Azide free (ab247427)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 nuclear extract lysate (ab14860)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/25000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt		1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		1/500.

Notes
WB 1/25000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

Application notes

Is unsuitable for IP.

Function

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
Sequence similarities

Belongs to the AP-2 family.
Domain

The WW-binding motif mediates interaction with WWOX.
Post-translational
modifications

Sumoylated on Lys-10; which inhibits transcriptional activity.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q92754 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7022 Human
- Omim: 601602 Human
- SwissProt: Q92754 Human
- Unigene: 473152 Human
Alternative names
- Activating enhancer binding protein 2 gamma antibody
- Activating enhancer-binding protein 2 gamma antibody
- AP2-gamma antibody
- AP2C_HUMAN antibody
- AP2g antibody
- AP2gamma antibody
- ERF 1 antibody
- ERF1 antibody
- Estrogen receptor factor 1 antibody
- hAP 2g antibody
- hAP2g antibody
- TFAP 2C antibody
- TFAP 2G antibody
- Tfap2c antibody
- TFAP2G antibody
- Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
- Transcription factor AP 2 gamma antibody
- Transcription factor AP-2 gamma antibody
- Transcription factor ERF 1 antibody
- Transcription factor ERF-1 antibody
- Transcription factor ERF1 antibody
see all

Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

All lanes : Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007) at 1/50000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : SKBR3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 49 kDa
Observed band size: 49 kDa
Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling AP2 gamma/TFAP2C with purified ab76007 at 1/500 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

ab76007 showing positive staining in Cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling AP2 gamma/TFAP2C with purified ab76007 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

ab76007 showing positive staining in Endometrial carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

ab76007 showing positive staining in Prostatic carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab76007? Please let us know so that we can cite the reference in this datasheet.

ab76007 has been referenced in 3 publications.

Wu VT et al. A TFAP2C Gene Signature Is Predictive of Outcome in HER2-Positive Breast Cancer. Mol Cancer Res 18:46-56 (2020). PubMed: 31619506
Chen D et al. The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation. Cell Rep 25:3591-3602.e5 (2018). PubMed: 30590035
Pastor WA et al. TFAP2C regulates transcription in human naive pluripotency by opening enhancers. Nat Cell Biol 20:553-564 (2018). PubMed: 29695788

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Human ESC)

Gel Running Conditions

Reduced Denaturing

Loading amount

500000 cells

Specification

Human ESC

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Timothy Hunt

Verified customer

Submitted Aug 22 2017

Question

Will this antibody cross react with AP2 alpha, beta, or epsilon?

Abcam community

Verified customer

Asked on Feb 22 2012

Answer

Thank you for your call today and for your question about ab76007.
I've heard back from the lab, and this antibody is not predicted to cross react with AP2 alpha, beta, or epsilon. The exact immunogen shares 60%, 60%, and ˜0% identity with these AP2 family members.
I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Abcam Scientific Support

Answered on Feb 22 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (3)

Customer reviews and Q&As

Western blot abreview for Anti-AP2 gamma antibody [EP2692Y]

Post-translational
modifications