Recombinant
RabMAb

Recombinant Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Reviews (1)Q&A (1)References (2)

Overview

  • Product name

    Anti-AP2 gamma/TFAP2C antibody [EP2692Y]
    See all AP2 gamma/TFAP2C primary antibodies

  • Description

    Rabbit monoclonal [EP2692Y] to AP2 gamma/TFAP2C

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human AP2 gamma/TFAP2C aa 100-200. The exact sequence is proprietary.

  • Positive control

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as AP2 gamma

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab76007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.

    • Sequence similarities

      Belongs to the AP-2 family.

    • Domain

      The WW-binding motif mediates interaction with WWOX.

    • Post-translational
      modifications

      Sumoylated on Lys-10; which inhibits transcriptional activity.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Activating enhancer binding protein 2 gamma antibody
        • Activating enhancer-binding protein 2 gamma antibody
        • AP2-gamma antibody
        • AP2C_HUMAN antibody
        • AP2g antibody
        • AP2gamma antibody
        • ERF 1 antibody
        • ERF1 antibody
        • Estrogen receptor factor 1 antibody
        • hAP 2g antibody
        • hAP2g antibody
        • TFAP 2C antibody
        • TFAP 2G antibody
        • Tfap2c antibody
        • TFAP2G antibody
        • Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
        • Transcription factor AP 2 gamma antibody
        • Transcription factor AP-2 gamma antibody
        • Transcription factor ERF 1 antibody
        • Transcription factor ERF-1 antibody
        • Transcription factor ERF1 antibody
        see all

      Images

      • Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
        Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
        All lanes : Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007) at 1/50000 dilution

        Lane 1 : MCF7 cell lysate
        Lane 2 : SKBR3 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/1000 dilution

        Predicted band size: 49 kDa
        Observed band size: 49 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
        Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

        Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling AP2 gamma/TFAP2C with purified ab76007 at 1/500 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

        ab76007 showing positive staining in Cervical carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

        ab76007 showing positive staining in Endometrial carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

        ab76007 showing positive staining in Prostatic carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      See all 2 Publications for this product

      Publishing research using ab76007? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-AP2 gamma antibody [EP2692Y]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human ESC)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      500000 cells
      Specification
      Human ESC
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Mr. Timothy Hunt

      Verified customer

      Submitted Aug 22 2017

      Question

      Will this antibody cross react with AP2 alpha, beta, or epsilon?

      Read More
      Answer

      Thank you for your call today and for your question about ab76007.
      I've heard back from the lab, and this antibody is not predicted to cross react with AP2 alpha, beta, or epsilon. The exact immunogen shares 60%, 60%, and ˜0% identity with these AP2 family members.
      I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

      Read More

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