Product nameAnti-AP2 gamma/TFAP2C antibody [EP2693Y]
See all AP2 gamma/TFAP2C primary antibodies
DescriptionRabbit monoclonal [EP2693Y] to AP2 gamma/TFAP2C
SpecificityThe immunogen used for this product shares 88% homology with cadherin EGF LAG seven-pass G-type receptor 1. Cross-reactivity with this protein has not been confirmed experimentally
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human AP2 gamma/TFAP2C aa 350-450 (C terminal). The exact sequence is proprietary.
- Purchase matching WB positive control:Recombinant Human AP2 gamma/TFAP2C protein
- MCF7 nuclear lysate Permeabilized MCF7 cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as AP2 gamma
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100000 - 1/200000. Detects a band of approximately 49 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
Sequence similaritiesBelongs to the AP-2 family.
DomainThe WW-binding motif mediates interaction with WWOX.
modificationsSumoylated on Lys-10; which inhibits transcriptional activity.
- Information by UniProt
- Activating enhancer binding protein 2 gamma antibody
- Activating enhancer-binding protein 2 gamma antibody
- AP2-gamma antibody
Anti-AP2 gamma/TFAP2C antibody [EP2693Y] (ab75992) at 1/200000 dilution + MCF-7 cell lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 49 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of permeabilized MCF-7cells using ab75992 (red) or a rabbit IgG (negative) (green).
ab75992 has been referenced in 1 publication.
- Cyr AR et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Human . PubMed: 24469049