Anti-AP2 gamma/TFAP2C antibody [EP2693Y] (ab75992)
Key features and details
- Rabbit monoclonal [EP2693Y] to AP2 gamma/TFAP2C
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-AP2 gamma/TFAP2C antibody [EP2693Y]
See all AP2 gamma/TFAP2C primary antibodies -
Description
Rabbit monoclonal [EP2693Y] to AP2 gamma/TFAP2C -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 88% homology with cadherin EGF LAG seven-pass G-type receptor 1. Cross-reactivity with this protein has not been confirmed experimentally
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Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF-7 lysate. Flow Cyt (Intra): MCF-7 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2693Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/100000 - 1/200000. Detects a band of approximately 49 kDa.
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Notes |
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Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/100000 - 1/200000. Detects a band of approximately 49 kDa. |
Target
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Function
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. -
Sequence similarities
Belongs to the AP-2 family. -
Domain
The WW-binding motif mediates interaction with WWOX. -
Post-translational
modificationsSumoylated on Lys-10; which inhibits transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7022 Human
- Omim: 601602 Human
- SwissProt: Q92754 Human
- Unigene: 473152 Human
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Alternative names
- Activating enhancer binding protein 2 gamma antibody
- Activating enhancer-binding protein 2 gamma antibody
- AP2-gamma antibody
see all
Images
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Anti-AP2 gamma/TFAP2C antibody [EP2693Y] (ab75992) at 1/200000 dilution + MCF-7 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 49 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of permeabilized MCF-7 cells using ab75992 (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab75992 has been referenced in 1 publication.
- Cyr AR et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Human . PubMed: 24469049