Recombinant Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20331] to AP2 gamma/TFAP2C
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AP2 gamma/TFAP2C antibody [EPR20331]
See all AP2 gamma/TFAP2C primary antibodies -
Description
Rabbit monoclonal [EPR20331] to AP2 gamma/TFAP2C -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SK-BR-3, MDA-MB-435S, MCF7 and T-47D whole cell lysates. IHC-P: Human breast, placenta, breast cancer and seminoma tissues. ICC/IF: SK-BR-3 and MCF7 cells. Flow Cyt (intra): SK-BR-3 cells. IP: MCF7 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20331 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/500.
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WB |
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/50.
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IP |
1/30.
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Notes |
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ICC/IF
1/500. |
WB
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/50. |
IP
1/30. |
Target
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Function
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. -
Sequence similarities
Belongs to the AP-2 family. -
Domain
The WW-binding motif mediates interaction with WWOX. -
Post-translational
modificationsSumoylated on Lys-10; which inhibits transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7022 Human
- Omim: 601602 Human
- SwissProt: Q92754 Human
- Unigene: 473152 Human
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Alternative names
- Activating enhancer binding protein 2 gamma antibody
- Activating enhancer-binding protein 2 gamma antibody
- AP2-gamma antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human breast tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Nuclear staining on human normal breast is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2C with ab218107 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on SK-BR-3 cell line.
The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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Lanes 1-2 : Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107) at 1/1000 dilution
Lanes 3-4 : Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107) at 1/2000 dilution
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate
Lane 2 : MDA-MB-435S (Human ductal carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 30 seconds; Lane 3: 10 seconds; Lane 4: 3 minutes.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2Cwith ab218107 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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AP2 gamma/TFAP2C was immunoprecipitated from 0.35 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab218107 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab218107 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: MCF7 whole cell lysate, 10 µg (Input).
Lane 2: ab218107 IP in MCF7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab2018107 in MCF7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Nuclear staining on human placenta is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Nuclear staining on poorly differentiated human breast cancer is observed. The data is consistent with the literature (PMID: 18825690).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2C with ab218107 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on MCF7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Nucleus staining on human seminoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Almost all tumor cells show negative staining on moderately differentiated human breast cancer. The data is consistent with the literature (PMID: 18825690).
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab218107 has been referenced in 8 publications.
- Fang F & Yuan Q Anlotinib inhibits the proliferation, migration and invasion, and induces apoptosis of breast cancer cells by downregulating TFAP2C. Oncol Lett 23:46 (2022). PubMed: 34976158
- Xu Y et al. Derivation of totipotent-like stem cells with blastocyst-like structure forming potential. Cell Res 32:513-529 (2022). PubMed: 35508506
- Huang H et al. Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment. Sci Adv 8:eabo5668 (2022). PubMed: 35857527
- Shang D et al. PGCLCs of human 45,XO reveal pathogenetic pathways of neurocognitive and psychosocial disorders. Cell Biosci 12:194 (2022). PubMed: 36457060
- Shao Y et al. An Original Ferroptosis-Related Gene Signature Effectively Predicts the Prognosis and Clinical Status for Colorectal Cancer Patients. Front Oncol 11:711776 (2021). PubMed: 34249766
- Bai T et al. Establishment of human induced trophoblast stem-like cells from term villous cytotrophoblasts. Stem Cell Res 56:102507 (2021). PubMed: 34454392
- Jin F et al. DNMT1-mediated methylation inhibits microRNA-214-3p and promotes hair follicle stem cell differentiate into adipogenic lineages. Stem Cell Res Ther 11:444 (2020). PubMed: 33076979
- Xiong G et al. MiR-10a-5p targets TFAP2C to promote gemcitabine resistance in pancreatic ductal adenocarcinoma. J Exp Clin Cancer Res 37:76 (2018). PubMed: 29615098