Overview

  • Product name

  • Description

    Rabbit polyclonal to AP2B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human AP2B1 aa 575-625. The exact sequence is proprietary. NP_001273.1
    Sequence:

    HKPPNA FVEGSHGIHR KHLPIHHGST DAGDSPVGTT TATNLEQPQV IPSQG


    Database link: P63010

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab205014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
  • Sequence similarities

    Belongs to the adaptor complexes large subunit family.
  • Post-translational
    modifications

    Phosphorylation at Tyr-737 by SRC occurs at the plasma membrane in clathrin-coated vesicles (CCVs).
  • Cellular localization

    Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adapter related protein complex 2 beta 1 subunit antibody
    • Adapter-related protein complex 2 beta subunit antibody
    • Adaptin, beta 2 (beta) antibody
    • Adaptor protein complex AP-2 subunit beta antibody
    • Adaptor related protein complex 2, beta 1 subunit antibody
    • ADTB2 antibody
    • AP-2 complex subunit beta antibody
    • AP105B antibody
    • AP2 BETA antibody
    • Ap2b1 antibody
    • AP2B1_HUMAN antibody
    • Beta adaptin antibody
    • Beta-2-adaptin antibody
    • Beta-adaptin antibody
    • Beta2 adaptin antibody
    • CLAPB1 antibody
    • Clathrin assembly protein complex 2 beta large chain antibody
    • Clathrin associated/assembly/adaptor protein, large, beta 1 antibody
    • DKFZp781K0743 antibody
    • Plasma membrane adaptor HA2/AP2 adaptin beta subunit antibody
    see all

Images

  • Detection of AP2B1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205014 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab205014 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.  Lysate prepared using NETN lysis buffer.

  • All lanes : Anti-AP2B1 antibody (ab205014) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 105 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer

References

ab205014 has not yet been referenced specifically in any publications.

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