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    ap2m1-antibody-ab137727.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Adapters
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Anti-AP2M1 antibody (ab137727)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Western blot - Anti-AP2M1 antibody (ab137727)
  • Western blot - Anti-AP2M1 antibody (ab137727)

Key features and details

  • Rabbit polyclonal to AP2M1
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-AP2M1 antibody
    See all AP2M1 primary antibodies
  • Description

    Rabbit polyclonal to AP2M1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human AP2M1 aa 60-268.

  • Positive control

    • HepG2 and Mouse brain whole cell lysates
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Adapters
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa.

Target

  • Function

    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling.
  • Sequence similarities

    Belongs to the adaptor complexes medium subunit family.
    Contains 1 MHD (mu homology) domain.
  • Cellular localization

    Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Target information above from: UniProt accession Q96CW1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 770246 Chicken
    • Entrez Gene: 517446 Cow
    • Entrez Gene: 1173 Human
    • Entrez Gene: 11773 Mouse
    • Entrez Gene: 116563 Rat
    • Entrez Gene: 380497 Xenopus laevis
    • Entrez Gene: 394572 Xenopus tropicalis
    • Omim: 601024 Human
    • SwissProt: Q5ZMP6 Chicken
    • SwissProt: Q3ZC13 Cow
    • SwissProt: Q96CW1 Human
    • SwissProt: P84091 Mouse
    • SwissProt: P84092 Rat
    • SwissProt: Q801Q8 Xenopus laevis
    • SwissProt: Q6P856 Xenopus tropicalis
    • Unigene: 518460 Human
    • Unigene: 18946 Mouse
    • Unigene: 3172 Rat
    see all
  • Alternative names

    • Adapter-related protein complex 2 mu subunit antibody
    • Adaptin mu 1 antibody
    • Adaptin-mu2 antibody
    • Adaptor protein complex AP 2 subunit mu antibody
    • Adaptor protein complex AP-2 subunit mu antibody
    • Adaptor related protein complex 2 mu 1 subunit antibody
    • AP 2 mu 2 chain antibody
    • AP-2 complex subunit mu antibody
    • AP-2 mu chain antibody
    • Ap2m1 antibody
    • AP2M1_HUMAN antibody
    • AP50 antibody
    • CLAPM1 antibody
    • Clathrin adaptor complex AP2 mu subunit antibody
    • Clathrin assembly protein complex 2 medium chain antibody
    • Clathrin associated/assembly/adaptor protein medium 1 antibody
    • Clathrin coat adaptor protein AP50 antibody
    • Clathrin coat assembly protein AP50 antibody
    • Clathrin coat associated protein AP50 antibody
    • Clathrin coat-associated protein AP50 antibody
    • HA2 50 kDa subunit antibody
    • mu2 antibody
    • Plasma membrane adaptor AP-2 50 kDa protein antibody
    see all

Images

  • Western blot - Anti-AP2M1 antibody (ab137727)
    Western blot - Anti-AP2M1 antibody (ab137727)
    Anti-AP2M1 antibody (ab137727) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Western blot - Anti-AP2M1 antibody (ab137727)
    Western blot - Anti-AP2M1 antibody (ab137727)
    Anti-AP2M1 antibody (ab137727) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 50 kDa



    10% SDS PAGE

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab137727? Please let us know so that we can cite the reference in this datasheet.

ab137727 has been referenced in 3 publications.

  • Mankouri J  et al. Release of Infectious Hepatitis C Virus from Huh7 Cells Occurs via a trans-Golgi Network-to-Endosome Pathway Independent of Very-Low-Density Lipoprotein Secretion. J Virol 90:7159-70 (2016). ICC/IF . PubMed: 27226379
  • Kakiuchi-Kiyota S  et al. Comparison of hepatic transcription profiles of locked ribonucleic acid antisense oligonucleotides: evidence of distinct pathways contributing to non-target mediated toxicity in mice. Toxicol Sci 138:234-48 (2014). WB ; Mouse . PubMed: 24336348
  • Hagemann AI  et al. In vivo analysis of formation and endocytosis of the Wnt/ß-catenin signaling complex in zebrafish embryos. J Cell Sci 127:3970-82 (2014). WB ; Zebrafish . PubMed: 25074807

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