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Signal Transduction Protein Trafficking Vesicle Transport Adapters
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RecombinantRabMAb

Recombinant Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)

  • Datasheet
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Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)
  • Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4700] to AP2M1 (phospho T156)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-AP2M1 (phospho T156) antibody [EPR4700]
    See all AP2M1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4700] to AP2M1 (phospho T156)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects AP2M1 phosphorylated at Threonine 156.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Threonine 156 of Human AP2 mu1 (Q96CW1)

  • Positive control

    • 293T cell lysate treated with calyculin A
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4700
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Adapters
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins

Associated products

  • Alternative Versions

    • Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (ab247848)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab109397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling.
    • Sequence similarities

      Belongs to the adaptor complexes medium subunit family.
      Contains 1 MHD (mu homology) domain.
    • Cellular localization

      Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
    • Target information above from: UniProt accession Q96CW1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1173 Human
      • Entrez Gene: 11773 Mouse
      • Entrez Gene: 116563 Rat
      • Omim: 601024 Human
      • SwissProt: Q96CW1 Human
      • SwissProt: P84091 Mouse
      • SwissProt: P84092 Rat
      • Unigene: 518460 Human
      • Unigene: 18946 Mouse
      • Unigene: 3172 Rat
      see all
    • Alternative names

      • Adapter-related protein complex 2 mu subunit antibody
      • Adaptin mu 1 antibody
      • Adaptin-mu2 antibody
      • Adaptor protein complex AP 2 subunit mu antibody
      • Adaptor protein complex AP-2 subunit mu antibody
      • Adaptor related protein complex 2 mu 1 subunit antibody
      • AP 2 mu 2 chain antibody
      • AP-2 complex subunit mu antibody
      • AP-2 mu chain antibody
      • Ap2m1 antibody
      • AP2M1_HUMAN antibody
      • AP50 antibody
      • CLAPM1 antibody
      • Clathrin adaptor complex AP2 mu subunit antibody
      • Clathrin assembly protein complex 2 medium chain antibody
      • Clathrin associated/assembly/adaptor protein medium 1 antibody
      • Clathrin coat adaptor protein AP50 antibody
      • Clathrin coat assembly protein AP50 antibody
      • Clathrin coat associated protein AP50 antibody
      • Clathrin coat-associated protein AP50 antibody
      • HA2 50 kDa subunit antibody
      • mu2 antibody
      • Plasma membrane adaptor AP-2 50 kDa protein antibody
      see all

    Images

    • Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)
      Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)
      All lanes : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : 293T cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)
      Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (5)

    Publishing research using ab109397? Please let us know so that we can cite the reference in this datasheet.

    ab109397 has been referenced in 5 publications.

    • Agajanian MJ  et al. WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop. Cell Rep 26:79-93.e8 (2019). PubMed: 30605688
    • Wrobel AG  et al. Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation. Dev Cell 50:494-508.e11 (2019). PubMed: 31430451
    • Wang C  et al. The Serine/Threonine Kinase AP2-Associated Kinase 1 Plays an Important Role in Rabies Virus Entry. Viruses 12:N/A (2019). PubMed: 31905947
    • Beacham GM  et al. NECAPs are negative regulators of the AP2 clathrin adaptor complex. Elife 7:N/A (2018). PubMed: 29345618
    • Hollopeter G  et al. The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex. Elife 3:N/A (2014). PubMed: 25303366

    Customer reviews and Q&As

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