Overview

  • Product name

    Anti-AP2S1 antibody [EPR2696]
    See all AP2S1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2696] to AP2S1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human AP2S1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Fetal brain lysate, HeLa whole cell lysate (ab150035)
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2696
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IP 1/10.
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
    • Sequence similarities

      Belongs to the adaptor complexes small subunit family.
    • Cellular localization

      Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
    • Information by UniProt
    • Database links

    • Alternative names

      • Adapter-related protein complex 2 sigma subunit antibody
      • Adaptor protein complex AP 2 subunit sigma antibody
      • Adaptor protein complex AP-2 subunit sigma antibody
      • Adaptor protein complex AP2 subunit sigma antibody
      • Adaptor related protein complex 2 sigma 1 subunit antibody
      • AI043088 antibody
      • AP 17 antibody
      • AP 2 complex subunit sigma 1 antibody
      • AP-2 complex subunit sigma antibody
      • AP17 antibody
      • AP17 delta antibody
      • AP2 complex subunit sigma 1 antibody
      • Ap2s1 antibody
      • AP2S1_HUMAN antibody
      • CLAPS 2 antibody
      • CLAPS2 antibody
      • Clathrin adaptor complex AP2, sigma subunit antibody
      • Clathrin adaptor protein AP17 antibody
      • Clathrin assembly protein 2 small chain antibody
      • Clathrin associated/assembly/adaptor protein small 2 antibody
      • Clathrin associated/assembly/adaptor protein small 2, 17-KD antibody
      • Clathrin associated/assembly/adaptor protein, small 2 (17kD) antibody
      • Clathrin coat assembly protein AP17 antibody
      • Clathrin coat associated protein AP17 antibody
      • Clathrin coat-associated protein AP17 antibody
      • HA2 17 kDa subunit antibody
      • MGC62945 antibody
      • Plasma membrane adaptor AP 2 17 kDa protein antibody
      • Plasma membrane adaptor AP-2 17 kDa protein antibody
      • Plasma membrane adaptor AP2 17 kDa protein antibody
      • Sigma adaptin 3b antibody
      • Sigma2 adaptin antibody
      • Sigma2-adaptin antibody
      see all

    Images

    • All lanes : Anti-AP2S1 antibody [EPR2696] (ab92380) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    References

    ab92380 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (endothelial)
    Specification
    endothelial
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton 0.1%
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jun 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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