RecombinantRabMAb

Recombinant Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
  • Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
  • Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2697(2)] to AP2S1 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free
    See all AP2S1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2697(2)] to AP2S1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human AP2S1 aa 100-200. The exact sequence is proprietary.
    Database link: P53680

  • Positive control

    • IHC-P: Human breast carcinoma, thyroid carcinoma and colon carcinoma tissues IP: Human fetal brain lysate

  • General notes

    Ab242393 is the carrier-free version of ab128950. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242393 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR2697(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab242393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

    • Sequence similarities

      Belongs to the adaptor complexes small subunit family.

    • Cellular localization

      Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adapter-related protein complex 2 sigma subunit antibody
      • Adaptor protein complex AP 2 subunit sigma antibody
      • Adaptor protein complex AP-2 subunit sigma antibody
      • Adaptor protein complex AP2 subunit sigma antibody
      • Adaptor related protein complex 2 sigma 1 subunit antibody
      • AI043088 antibody
      • AP 17 antibody
      • AP 2 complex subunit sigma 1 antibody
      • AP-2 complex subunit sigma antibody
      • AP17 antibody
      • AP17 delta antibody
      • AP2 complex subunit sigma 1 antibody
      • Ap2s1 antibody
      • AP2S1_HUMAN antibody
      • CLAPS 2 antibody
      • CLAPS2 antibody
      • Clathrin adaptor complex AP2, sigma subunit antibody
      • Clathrin adaptor protein AP17 antibody
      • Clathrin assembly protein 2 small chain antibody
      • Clathrin associated/assembly/adaptor protein small 2 antibody
      • Clathrin associated/assembly/adaptor protein small 2, 17-KD antibody
      • Clathrin associated/assembly/adaptor protein, small 2 (17kD) antibody
      • Clathrin coat assembly protein AP17 antibody
      • Clathrin coat associated protein AP17 antibody
      • Clathrin coat-associated protein AP17 antibody
      • HA2 17 kDa subunit antibody
      • MGC62945 antibody
      • Plasma membrane adaptor AP 2 17 kDa protein antibody
      • Plasma membrane adaptor AP-2 17 kDa protein antibody
      • Plasma membrane adaptor AP2 17 kDa protein antibody
      • Sigma adaptin 3b antibody
      • Sigma2 adaptin antibody
      • Sigma2-adaptin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1:100 dilution (12.07 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
      Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

      ab128950 (purified) at 1:60 dilution (2µg) immunoprecipitating AP2S1 in Human fetal brain lysate.
      Lane 1 (input): Human fetal brain lysate 10µg
      Lane 2 (+): ab128950 & Human fetal brain lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128950 in Human fetal brain lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

      ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128950).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

      ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded human colon carcinoma tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128950).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1:100 dilution (12.07 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)
      Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab242393? Please let us know so that we can cite the reference in this datasheet.

    ab242393 has not yet been referenced specifically in any publications.

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