Overview

  • Product name

    Anti-AP3M1 antibody [EPR16385]
    See all AP3M1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16385] to AP3M1
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product(ab201227) shares 78% homologue with AP3M2. (AP-3 complex subunit mu-2). Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AP3M1 aa 100-200. The exact sequence is proprietary.
    Database link: Q9Y2T2

  • Positive control

    • WB: HeLa and HEK293 cell lysates; Human fetal brain lysate. ICC/IF: HeLa cells Flow Cyt: HEK293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF 1/250.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
  • Sequence similarities

    Belongs to the adaptor complexes medium subunit family.
    Contains 1 MHD (mu homology) domain.
  • Cellular localization

    Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adapter-related protein complex 3 mu-1 subunit antibody
    • AP-3 adapter complex mu3A subunit antibody
    • AP-3 complex subunit mu-1 antibody
    • AP3M1 antibody
    • AP3M1_HUMAN antibody
    • Clathrin adaptor complex AP3 mu 3A subunit antibody
    • Mu-adaptin 3A antibody
    • mu3A antibody
    • Mu3A-adaptin antibody
    see all

Images

  • All lanes : Anti-AP3M1 antibody [EPR16385] (ab201227) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK293 (Human embryonic kidney) cell lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling AP3M1 with ab201227 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab201227 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HEK293 (Human embryonic kidney) cells labeling AP3M1 with ab201227 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Karampini E  et al. Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells. Haematologica N/A:N/A (2019). Read more (PubMed: 30630984) »
  • Gonçalves E  et al. Widespread Post-transcriptional Attenuation of Genomic Copy-Number Variation in Cancer. Cell Syst 5:386-398.e4 (2017). Read more (PubMed: 29032074) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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