Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16385] to AP3M1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-AP3M1 antibody [EPR16385]
See all AP3M1 primary antibodies
DescriptionRabbit monoclonal [EPR16385] to AP3M1
The immunogen used for this product(ab201227) shares 78% homologue with AP3M2. (AP-3 complex subunit mu-2). Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human AP3M1 aa 100-200. The exact sequence is proprietary.
Database link: Q9Y2T2
- WB: HeLa and HEK293 cell lysates; Human fetal brain lysate. ICC/IF: HeLa cells Flow Cyt: HEK293 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab201227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPart of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
Sequence similaritiesBelongs to the adaptor complexes medium subunit family.
Contains 1 MHD (mu homology) domain.
Cellular localizationGolgi apparatus.
- Information by UniProt
- Adapter-related protein complex 3 mu-1 subunit antibody
- AP-3 adapter complex mu3A subunit antibody
- AP-3 complex subunit mu-1 antibody
All lanes : Anti-AP3M1 antibody [EPR16385] (ab201227) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 2 : HEK293 (Human embryonic kidney) cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 10 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling AP3M1 with ab201227 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab201227 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed HEK293 (Human embryonic kidney) cells labeling AP3M1 with ab201227 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
ab201227 has been referenced in 2 publications.
- Karampini E et al. Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells. Haematologica N/A:N/A (2019). PubMed: 30630984
- Gonçalves E et al. Widespread Post-transcriptional Attenuation of Genomic Copy-Number Variation in Cancer. Cell Syst 5:386-398.e4 (2017). PubMed: 29032074