Product nameAnti-AP3M2 antibody [EPR12965] - N-terminal
See all AP3M2 primary antibodies
DescriptionRabbit monoclonal [EPR12965] to AP3M2 - N-terminal
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human AP3M2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P53677
- Human fetal brain, HepG2, SH-SY5Y and U87-MG lysates; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevancePart of the AP-3 complex, AP3M3 is an adaptor-related complex not clathrin-associated. The complex is associated with the Golgi region and to more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
Cellular localizationGolgi apparatus.
- Adapter related protein complex 3 mu 2 subunit antibody
- AP-3 complex subunit mu 2 antibody
- Clathrin assembly protein assembly protein complex 1 medium chain homolog 2 antibody
All lanes : Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138) at 1/20000 dilution
Lane 1 : Human fetal brain Lysate
Lane 2 : HepG2 cell Lysate
Lane 3 : SH-SY5Y cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling AP3M2 with ab180138 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 488) at 1/250 dilution. Conterstained with Dapi blue.
Flow cytometric analysis of 2% paraformaldehyde fixed U87-MG cells labeling AP3M2 with ab180138 at 1/30 dilution. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.
Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138) at 1/5000 dilution + U87-MG at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa
ab180138 has not yet been referenced specifically in any publications.