Product nameAnti-AP3M2 antibody [EPR12966]
See all AP3M2 primary antibodies
DescriptionRabbit monoclonal [EPR12966] to AP3M2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AP3M2 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: P53677
- HepG2, U87-MG and SH-SY5Y cell lysates; Human brain tissue; SH-SY5Y cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevancePart of the AP-3 complex, AP3M3 is an adaptor-related complex not clathrin-associated. The complex is associated with the Golgi region and to more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
Cellular localizationGolgi apparatus.
- Adapter related protein complex 3 mu 2 subunit antibody
- AP-3 complex subunit mu 2 antibody
- Clathrin assembly protein assembly protein complex 1 medium chain homolog 2 antibody
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling AP3M2 with ab182000 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
All lanes : Anti-AP3M2 antibody [EPR12966] (ab182000) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution
Predicted band size: 47 kDa
Flow cytometric analysis of SH-SY5Y cells (paraformaldehyde-fixed, 2%) labeling AP3M2 with ab182000 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
ab182000 has not yet been referenced specifically in any publications.