Recombinant
RabMAb

Recombinant Anti-AP3M2 antibody [EPR12966] (ab182000)

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Overview

  • Product name

    Anti-AP3M2 antibody [EPR12966]
    See all AP3M2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12966] to AP3M2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AP3M2 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P53677

  • Positive control

    • HepG2, U87-MG and SH-SY5Y cell lysates; Human brain tissue; SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab182000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    Part of the AP-3 complex, AP3M3 is an adaptor-related complex not clathrin-associated. The complex is associated with the Golgi region and to more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

  • Cellular localization

    Golgi apparatus.

  • Database links

    • Alternative names

      • Adapter related protein complex 3 mu 2 subunit antibody
      • AP-3 complex subunit mu 2 antibody
      • Clathrin assembly protein assembly protein complex 1 medium chain homolog 2 antibody
      • Clathrin coat assembly protein AP47 homolog 2 antibody
      • Clathrin coat associated protein AP47 homolog 2 antibody
      • Golgi adaptor AP1 47 kDa protein homolog 2 antibody
      • HA1 47 kDa subunit homolog 2 antibody
      • Mu3B adaptin antibody
      • P47B antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP3M2 antibody [EPR12966] (ab182000)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP3M2 antibody [EPR12966] (ab182000)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling AP3M2 with ab182000 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-AP3M2 antibody [EPR12966] (ab182000)
      Western blot - Anti-AP3M2 antibody [EPR12966] (ab182000)
      All lanes : Anti-AP3M2 antibody [EPR12966] (ab182000) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

      Predicted band size: 47 kDa

    • Flow Cytometry - Anti-AP3M2 antibody [EPR12966] (ab182000)
      Flow Cytometry - Anti-AP3M2 antibody [EPR12966] (ab182000)

      Flow cytometric analysis of SH-SY5Y cells (paraformaldehyde-fixed, 2%) labeling AP3M2 with ab182000 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

    References

    Publishing research using ab182000? Please let us know so that we can cite the reference in this datasheet.

    ab182000 has not yet been referenced specifically in any publications.

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