- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-AP3S1 antibody
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DescriptionRabbit polyclonal to AP3S1
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Host speciesRabbit
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Specificityab113099 will detect only the two larger AP3S1 isoforms. ab113099 is not predicted to cross-react with other AP3 protein family members.
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Tested applicationsSuitable for: WB, ICC/IF, IHC-P, ICCmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide containing a 19 amino acid sequence from internal residues of Human AP3S1 (NP_001275).
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Positive control
- Mouse kidney tissue lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab113099 is purified by affinity chromatography via a peptide column.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab113099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 22 kDa. | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC | Use a concentration of 2.5 µg/ml. |
Target
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FunctionPart of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
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Tissue specificityPresent in all adult tissues examined.
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Sequence similaritiesBelongs to the adaptor complexes small subunit family.
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Cellular localizationGolgi apparatus. Cytoplasmic vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
- Information by UniProt
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Database links
- Entrez Gene: 1176 Human
- Entrez Gene: 11777 Mouse
- Entrez Gene: 302290 Rat
- Omim: 601507 Human
- SwissProt: Q92572 Human
- SwissProt: Q9DCR2 Mouse
- Unigene: 728956 Human
- Unigene: 312536 Mouse
see all -
Alternative names
- Adapter-related protein complex 3 sigma-1 subunit antibody
- Adaptor-related protein complex 3 antibody
- AP-3 complex sigma-3A subunit antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AP3S1 antibody (ab113099)Immunofluorescence of AP3S1 in Human kidney tissue with ab113099 at 20 ug/mL. -
Western blot - Anti-AP3S1 antibody (ab113099)Lane 1 : Anti-AP3S1 antibody (ab113099) at 1 µg/ml
Lane 2 : Anti-AP3S1 antibody (ab113099) at 2 µg/ml
All lanes : Mouse kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa -
Immunohistochemistry - Anti-AP3S1 antibody (ab113099)Immunohistochemistry of AP3S1 in human kidney tissue with ab113099 at 2.5 ug/mL.
Protocols
References
ab113099 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"