Anti-AP3S1 antibody (ab113099)
Key features and details
- Rabbit polyclonal to AP3S1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AP3S1 antibody -
Description
Rabbit polyclonal to AP3S1 -
Host species
Rabbit -
Specificity
ab113099 will detect only the two larger AP3S1 isoforms. ab113099 is not predicted to cross-react with other AP3 protein family members. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide containing a 19 amino acid sequence from internal residues of Human AP3S1 (NP_001275).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab113099 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 22 kDa. | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. -
Tissue specificity
Present in all adult tissues examined. -
Sequence similarities
Belongs to the adaptor complexes small subunit family. -
Cellular localization
Golgi apparatus. Cytoplasmic vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 1176 Human
- Entrez Gene: 11777 Mouse
- Entrez Gene: 302290 Rat
- Omim: 601507 Human
- SwissProt: Q92572 Human
- SwissProt: Q9DCR2 Mouse
- Unigene: 728956 Human
- Unigene: 312536 Mouse
see all -
Alternative names
- Adapter-related protein complex 3 sigma-1 subunit antibody
- Adaptor-related protein complex 3 antibody
- AP-3 complex sigma-3A subunit antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AP3S1 antibody (ab113099)Immunofluorescence of AP3S1 in Human kidney tissue with ab113099 at 20 ug/mL.
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Western blot - Anti-AP3S1 antibody (ab113099)Lane 1 : Anti-AP3S1 antibody (ab113099) at 1 µg/ml
Lane 2 : Anti-AP3S1 antibody (ab113099) at 2 µg/ml
All lanes : Mouse kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP3S1 antibody (ab113099)Immunohistochemistry of AP3S1 in human kidney tissue with ab113099 at 2.5 ug/mL.
Protocols
Datasheets and documents
References (0)
ab113099 has not yet been referenced specifically in any publications.
