Anti-AP3S1 antibody (ab113099)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-AP3S1 antibody -
Description
Rabbit polyclonal to AP3S1 -
Host species
Rabbit -
Specificity
ab113099 will detect only the two larger AP3S1 isoforms. ab113099 is not predicted to cross-react with other AP3 protein family members. -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide containing a 19 amino acid sequence from internal residues of Human AP3S1 (NP_001275).
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Positive control
- Mouse kidney tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab113099 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 22 kDa. | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC | Use a concentration of 2.5 µg/ml. |
Target
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Function
Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. -
Tissue specificity
Present in all adult tissues examined. -
Sequence similarities
Belongs to the adaptor complexes small subunit family. -
Cellular localization
Golgi apparatus. Cytoplasmic vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 1176 Human
- Entrez Gene: 11777 Mouse
- Entrez Gene: 302290 Rat
- Omim: 601507 Human
- SwissProt: Q92572 Human
- SwissProt: Q9DCR2 Mouse
- Unigene: 728956 Human
- Unigene: 312536 Mouse
see all -
Alternative names
- Adapter-related protein complex 3 sigma-1 subunit antibody
- Adaptor-related protein complex 3 antibody
- AP-3 complex sigma-3A subunit antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AP3S1 antibody (ab113099)Immunofluorescence of AP3S1 in Human kidney tissue with ab113099 at 20 ug/mL. -
Western blot - Anti-AP3S1 antibody (ab113099)Lane 1 : Anti-AP3S1 antibody (ab113099) at 1 µg/ml
Lane 2 : Anti-AP3S1 antibody (ab113099) at 2 µg/ml
All lanes : Mouse kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP3S1 antibody (ab113099)Immunohistochemistry of AP3S1 in human kidney tissue with ab113099 at 2.5 ug/mL.
Protocols
Datasheets and documents
References
ab113099 has not yet been referenced specifically in any publications.
