Key features and details
- Rabbit polyclonal to AP3S1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-AP3S1 antibody
DescriptionRabbit polyclonal to AP3S1
Specificityab113099 will detect only the two larger AP3S1 isoforms. ab113099 is not predicted to cross-react with other AP3 protein family members.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide containing a 19 amino acid sequence from internal residues of Human AP3S1 (NP_001275).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab113099 is purified by affinity chromatography via a peptide column.
Our Abpromise guarantee covers the use of ab113099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 22 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
FunctionPart of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
Tissue specificityPresent in all adult tissues examined.
Sequence similaritiesBelongs to the adaptor complexes small subunit family.
Cellular localizationGolgi apparatus. Cytoplasmic vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
- Information by UniProt
- Adapter-related protein complex 3 sigma-1 subunit antibody
- Adaptor-related protein complex 3 antibody
- AP-3 complex sigma-3A subunit antibody
Immunofluorescence of AP3S1 in Human kidney tissue with ab113099 at 20 ug/mL.
Lane 1 : Anti-AP3S1 antibody (ab113099) at 1 µg/ml
Lane 2 : Anti-AP3S1 antibody (ab113099) at 2 µg/ml
All lanes : Mouse kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa
Immunohistochemistry of AP3S1 in human kidney tissue with ab113099 at 2.5 ug/mL.
ab113099 has not yet been referenced specifically in any publications.