Anti-AP4B1 antibody (ab235539)
Key features and details
- Rabbit polyclonal to AP4B1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AP4B1 antibody
See all AP4B1 primary antibodies -
Description
Rabbit polyclonal to AP4B1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human AP4B1 aa 200-500.
Database link: Q9Y6B7-1 -
Positive control
- WB: HepG2, U-251 MG and L02 whole cell lysate. IHC-P: Human testis and adrenal gland tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/2000. |
IHC-P
1/20 - 1/200. |
Target
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Relevance
Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. -
Cellular localization
Golgi apparatus, trans-Golgi network. -
Database links
- Entrez Gene: 10717 Human
- Entrez Gene: 67489 Mouse
- Omim: 607245 Human
- SwissProt: Q9Y6B7 Human
- SwissProt: Q9WV76 Mouse
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Alternative names
- Adapter related protein complex 4 beta 1 subunit antibody
- AP 4 adapter complex subunit beta antibody
- AP 4 complex subunit beta 1 antibody
- Beta subunit of AP 4 antibody
Images
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All lanes : Anti-AP4B1 antibody (ab235539) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) whole cell lysate
Lane 3 : L02 (Human immortal liver cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution -
Paraffin-embedded human testis tissue stained for AP4B1 with ab235539 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human adrenal gland tissue stained for AP4B1 with ab235539 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235539 has not yet been referenced specifically in any publications.