Key features and details
- Rabbit polyclonal to AP4B1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AP4B1 antibody
DescriptionRabbit polyclonal to AP4B1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human AP4B1 aa 201-500.
HHLLNRMSKLDQWGQAEVLNFLLRYQPRSEEELFDILNLLDSFLKSSSPG VVMGATKLFLILAKMFPHVQTDVLVRVKGPLLAACSSESRELCFVALCHV RQILHSLPGHFSSHYKKFFCSYSEPHYIKLQKVEVLCELVNDENVQQVLE ELRGYCTDVSADFAQAAIFAIGGIARTYTDQCVQILTELLGLRQEHITTV VVQTFRDLVWLCPQCTEAVCQALPGCEENIQDSEGKQALIWLLGVHGERI PNAPYVLEDFVENVKSETFPAVKMELLTALLRLFLSRPAECQDMLGRLLY
Database link: Q9Y6B7-1
- WB: HepG2, U-251 MG and L02 whole cell lysate. IHC-P: Human testis and adrenal gland tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|IHC-P||1/20 - 1/200.|
RelevanceSubunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.
Cellular localizationGolgi apparatus, trans-Golgi network.
- Adapter related protein complex 4 beta 1 subunit antibody
- AP 4 adapter complex subunit beta antibody
- AP 4 complex subunit beta 1 antibody
- Beta subunit of AP 4 antibody
All lanes : Anti-AP4B1 antibody (ab235539) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) whole cell lysate
Lane 3 : L02 (Human immortal liver cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Paraffin-embedded human testis tissue stained for AP4B1 with ab235539 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for AP4B1 with ab235539 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235539 has not yet been referenced specifically in any publications.