Overview

  • Product name

    Anti-AP4M1 antibody
  • Description

    Rabbit polyclonal to AP4M1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within the internal sequence amino acids 70-265 of Human AP4M1.

  • Positive control

    • 293T, A431, Jurkat, Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab96306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.

Target

  • Function

    Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.
  • Tissue specificity

    Ubiquitous. Highly expressed in testis and lowly expressed in brain and lung.
  • Involvement in disease

    Defects in AP4M1 are the cause of cerebral palsy spastic quadriplegic type 3 (CPSQ3) [MIM:612936]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals present postnatally with early infantile hypotonia, delayed psychomotor development, strabismus, lack of independent walking and severe mental retardation. They develop progressive spasticity of all limbs with generalized hypertonia, hyperreflexia, and extensor plantar responses by the end of the first year of life. Speech is absent or limited. Pseudobulbar signs, such as drooling, stereotypic laughter, and exaggerated jaw jerk, are part of the clinical picture.
  • Sequence similarities

    Belongs to the adaptor complexes medium subunit family.
    Contains 1 MHD (mu homology) domain.
  • Domain

    Interacts specifically with tyrosine-based sorting signals.
  • Cellular localization

    Golgi apparatus > trans-Golgi network. Membrane > coated pit. Associated with the trans-Golgi network. Found in soma and dendritic shafts of neuronal cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adapter-related protein complex 4 mu-1 subunit antibody
    • Adaptor related protein complex 4 mu 1 subunit antibody
    • Adaptor related protein complex AP 4 mu4 subunit antibody
    • Adaptor related protein complex AP4 mu4 subunit antibody
    • Adaptor-related protein complex AP-4, mu 1 antibody
    • AP 4 adapter complex mu subunit antibody
    • AP 4 complex subunit mu 1 antibody
    • AP-4 adapter complex mu subunit antibody
    • AP-4 complex subunit mu-1 antibody
    • AP4 adapter complex mu subunit antibody
    • AP4 complex subunit mu 1 antibody
    • AP4M1 antibody
    • AP4M1_HUMAN antibody
    • Ap4m4 antibody
    • CPSQ3 antibody
    • MU 4 antibody
    • Mu adaptin related protein 2 antibody
    • MU ARP2 antibody
    • Mu subunit of AP 4 antibody
    • Mu subunit of AP-4 antibody
    • Mu-adaptin-related protein 2 antibody
    • mu-ARP2 antibody
    • mu4 antibody
    • Mu4-adaptin antibody
    • MUARP 2 antibody
    • MUARP2 antibody
    • SPG50 antibody
    see all

Images

  • Anti-AP4M1 antibody (ab96306) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE

References

This product has been referenced in:

  • Contu VR  et al. Lysosomal targeting of SIDT2 via multiple YxxF motifs is required for SIDT2 function in the process of RNautophagy. J Cell Sci 130:2843-2853 (2017). Read more (PubMed: 28724756) »
  • Toh WH  et al. Amyloid precursor protein traffics from the Golgi directly to early endosomes in an Arl5b- and AP4-dependent pathway. Traffic 18:159-175 (2017). Read more (PubMed: 28000370) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (E16 hippocampal culture)
Permeabilization
Yes - MeOH+Tx-100
Specification
E16 hippocampal culture
Blocking step
0.5% BSA + 10% Horse Serum in PBS-Tx100 (0.5%) as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: 22°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted May 10 2017

Answer

Thanks for your patience.

Sorry for the confusion regarding the label of ab96306. I have confirmed that this antibody is not specific for phosphorylated AP4M1. It will detect total AP4M1.

Thanks for bringing this to our attention. Please note that the information for this product has been updated on our online product datasheet.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

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