Anti-APAF1 antibody (ab2000)
Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APAF1 antibody
See all APAF1 primary antibodies -
Description
Rabbit polyclonal to APAF1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human APAF1 aa 1158-1177 (C terminal).
Sequence:TFYTNGTNLKKIHVSPDFKT
(Peptide available asab7872) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 20 µg/ml.
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 130 kDa.
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Notes |
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IHC-P
Use a concentration of 20 µg/ml. |
WB
1/500 - 1/2000. Predicted molecular weight: 130 kDa. |
Target
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Function
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
Tissue specificity
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
Domain
The CARD domain mediates interaction with APIP. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 317 Human
- Omim: 602233 Human
- SwissProt: O14727 Human
- Unigene: 728891 Human
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Alternative names
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
Images
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All lanes : Anti-APAF1 antibody (ab2000) at 1 µg/ml
Lane 1 : Human heart tissue lysate with absence of blocking peptide
Lane 2 : Human heart tissue lysate with presence of blocking peptide
Predicted band size: 130 kDa
Observed band size: 130 kDa -
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab2000 has been referenced in 12 publications.
- Gui C et al. Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF-7 cell line. Int J Oncol 58:409-418 (2021). PubMed: 33469684
- Eggers B et al. Influences of cold atmospheric plasma on apoptosis related molecules in osteoblast-like cells in vitro. Head Face Med 17:37 (2021). PubMed: 34479596
- Zhang X et al. The effects of umbilical cord-derived macrophage exosomes loaded with cisplatin on the growth and drug resistance of ovarian cancer cells. Drug Dev Ind Pharm N/A:1-13 (2020). PubMed: 32482115
- Shao S et al. Toosendanin induces apoptosis of MKN-45 human gastric cancer cells partly through miR-23a-3p-mediated downregulation of BCL2. Mol Med Rep 22:1793-1802 (2020). PubMed: 32582989
- Hu X et al. The Anti-tumor Effects of p-Coumaric Acid on Melanoma A375 and B16 Cells. Front Oncol 10:558414 (2020). PubMed: 33178586