Overview

  • Product name

  • Description

    Rabbit polyclonal to APAF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFrmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human APAF1 aa 1200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O14727

  • Positive control

    • Testis

Properties

Applications

Our Abpromise guarantee covers the use of ab46899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/200.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function

    Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
  • Tissue specificity

    Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 NB-ARC domain.
    Contains 13 WD repeats.
  • Domain

    The CARD domain mediates interaction with APIP.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • APAF 1 antibody
    • Apaf-1 antibody
    • APAF_HUMAN antibody
    • Apaf1 antibody
    • Apoptotic peptidase activating factor 1 antibody
    • Apoptotic protease activating factor 1 antibody
    • Apoptotic protease activating factor antibody
    • Apoptotic protease-activating factor 1 antibody
    • CED 4 antibody
    • CED4 antibody
    • KIAA0413 antibody
    see all

Images

  • Human testis stained with ab46899 using the antibody at a dilution of 1:200

References

ab46899 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab46899.
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