Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-FoFr
- Reacts with: Human
- Isotype: IgG
Product nameAnti-APAF1 antibody
See all APAF1 primary antibodies
DescriptionRabbit polyclonal to APAF1
Tested applicationsSuitable for: IHC-FoFrmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human APAF1 aa 1200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O14727
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab46899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
FunctionOligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Tissue specificityUbiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Sequence similaritiesContains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.
DomainThe CARD domain mediates interaction with APIP.
- Information by UniProt
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab46899 has not yet been referenced specifically in any publications.