Overview

  • Product name
  • Description
    Rabbit polyclonal to APAF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Positive control
    • Testis

Properties

Applications

Our Abpromise guarantee covers the use of ab46899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
  • Application notes
    IHC-Fo: 1/200.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM
    Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
    • Tissue specificity
      Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
    • Sequence similarities
      Contains 1 CARD domain.
      Contains 1 NB-ARC domain.
      Contains 13 WD repeats.
    • Domain
      The CARD domain mediates interaction with APIP.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • APAF 1 antibody
      • Apaf-1 antibody
      • APAF_HUMAN antibody
      • Apaf1 antibody
      • Apoptotic peptidase activating factor 1 antibody
      • Apoptotic protease activating factor 1 antibody
      • Apoptotic protease activating factor antibody
      • Apoptotic protease-activating factor 1 antibody
      • CED 4 antibody
      • CED4 antibody
      • KIAA0413 antibody
      see all

    Images

    • Human testis stained with ab46899 using the antibody at a dilution of 1:200

    References

    ab46899 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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