Recombinant
RabMAb

Recombinant Anti-APC antibody [EP701Y] (ab40778)

Overview

  • Product name
    Anti-APC antibody [EP701Y]
    See all APC primary antibodies
  • Description
    Rabbit monoclonal [EP701Y] to APC
  • Host species
    Rabbit
  • Specificity
    This antibody is predicted to detect isoform 2 (short) of APC based on sequence analysis.
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human APC. The amino acid sequence is not available.

  • Positive control
    • 293 cell lysate, human colon carcinoma.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 160 kDa.
IHC-P 1/50 - 1/100.
IP 1/50.
Flow Cyt 1/130 - 1/150.
ICC 1/50 - 1/100.

Target

  • Function
    Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
  • Tissue specificity
    Expressed in a variety of tissues.
  • Involvement in disease
    Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years.
    Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also known as familial infiltrative fibromatosis (FIF). HDD is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis.
    Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome).
    Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) [MIM:276300]; also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
    Defects in APC are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
    Defects in APC are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. This defect includes also the disease entity termed hepatoblastoma.
  • Sequence similarities
    Belongs to the adenomatous polyposis coli (APC) family.
    Contains 7 ARM repeats.
  • Domain
    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    modifications
    Phosphorylated by GSK3B.
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
  • Cellular localization
    Cell junction > adherens junction. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle membrane. Cytoplasm. Cell membrane. Associated with the microtubule network at the growing distal tip of microtubules. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenomatous Polyposis Coli antibody
    • Adenomatous polyposis coli protein antibody
    • Apc antibody
    • APC_HUMAN antibody
    • CC1 antibody
    • Deleted in polyposis 2.5 antibody
    • DP2 antibody
    • DP2.5 antibody
    • DP3 antibody
    • FAP antibody
    • FPC antibody
    • GS antibody
    • Protein APC antibody
    see all

Images

  • ab40778 (1:50), staining human colon carcinoma by immunohistochemistry, paraffin-embedded tissue.

  • ab40778 staining APC in the human cell line 293 (human embryonic kidney) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Anti-APC antibody [EP701Y] (ab40778) at 1/1000 dilution + 10 ug/ml HEK293 cell lysate

    Secondary
    1:2000 goat anti-rabbit HRP labeled


    Observed band size: 160 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 250 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Juhlin CC  et al. Parafibromin immunostainings of parathyroid tumors in clinical routine: a near-decade experience from a tertiary center. Mod Pathol N/A:N/A (2019). Read more (PubMed: 30923346) »
  • Guo F  et al. The action mechanism of lncRNA-HOTAIR on the drug resistance of non-small cell lung cancer by regulating Wnt signaling pathway. Exp Ther Med 15:4885-4889 (2018). Read more (PubMed: 29805510) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Q&A

Answer

The exact immunogen shares 100% identity with bovine APC, so the antibody is
predicted to cross-react with bovine protein, although we have not tested this reactivity yet.
I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question

I believe what caused this e-mail exchange between us in the first place is your data sheet. First of all, your data sheets are usually flawless - with recommended dilutions, gel and IHC pictures and so forth which most companies exclude from theirs. However, this data sheet was a bit confusing regarding your WB image using the EP701Y antibody. We have managed to get three bands, two closely linked at 300 kDa, of which one is slightly bigger than the other in size, as well as the 160 kDa band. Your data sheet suggest that the "expected band size" of APC using this anitbody is 160 kDa. When I looked it up in the litterature, we figured that your antibody is targeting exon 3 of human APC, hence discovering NOT ONLY this unknown APC splice variant, but ALSO full length APC as well as isoform 2 (short) of 304 kDa. So I think you need to state this more clearly, since I was worried your antibody was ONLY detecting isoform 2 (short) which made no sense to me after reviewing the litterature. I can e-mail you the WB used with longer running time to separate the 300 kDa isoforms in higher resolution, but first after we have published the data. So, you have quite a precious antibody which detects all three forms of APC - and hence the loss of all three in tumor material would suggest certain inactivation patterns that is not possible using antibodies directing at other parts of APC, thus missing out on info regarding the 160 kDa variant - with unknown properties. I think it would be wise of you to state this, but that is merely the opinion of one young and fairly unexperienced researcher.Best regards, looking forward to more collaborations in the future,

Read More
Answer

Thank you for your valuable feedback. We will take into consideration the things you have mentioned and will keep other customers up to date as well. We look forward to future collaborations with you and good luck in your research.

Read More

Answer

Thank you for getting back to me. I am glad to hear that you managed to obtain satisfactory results with this product. I have checked the datasheets of other companies supplying this clone and it seems that our information are similar. Please forgive me for I do not know which part of our datasheet that is confusing? Is it the part that states the molecular weight to be 160kDa? Although this product is expected to detect the isoform 2 based on sequence analysis, but the actual band sizes that we obtain was around 160kDa and 250kDa. This 160kDa band is a spliced variant and we are not sure about the 250kDa band. This product is one of our good sellers and we have not received any complaints. If there is any information that you think is misleading, I would certainly like to hear more about it from you. If you should need anything else, please do not hesitate to contact me. Have a nice day.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up