Immunocytochemistry abreview for Anti-APC antibody [EP701Y]

Average
Abreviews
Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous HeLa cells were methanol-fixed, immunofluorescently labeled and counterstained with DAPI. The antibody produces the expected staining pattern, albeit somewhat weaker than other APC antibodies. Paraformaldehyde-fixed samples were also evaluated and produced a predominantly nuclear, focal staining pattern.

Dr. Kirk Mcmanus

Verified customer

Submitted Sep 07 2012

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