Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-APC antibody [EP701Y]

Below Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mammary tumor)
Specification
mammary tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Biotinylated Goat Anti-Rabbit IgG Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/2000

Additional data

Additional Notes
Counterstain was hematoxylin
Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody has not worked in your hands. Mouse is not a validated species for this antibody and your data suggest it may not be suitable for this species under the conditions in which you used. Many thanks for your feedback on this antibody, we hope that this information will be helpful for future customers.

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Verified customer

Submitted Feb 11 2013

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