APC Anti-CD14 antibody [MEM-15] (ab60901)
Key features and details
- APC Mouse monoclonal [MEM-15] to CD14
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD14 antibody [MEM-15]
See all CD14 primary antibodies -
Description
APC Mouse monoclonal [MEM-15] to CD14 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Specificity
This antibody reacts with CD14, a membrane bound glycoprotein. The antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
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Positive control
- Human blood cells
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General notes
Avoid prolonged exposure to light. Short-term exposure to room temperature should not affect the quality of the reagent.The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 1% BSA
0.2% high-grade protease free BSA -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP/Cy5.5® Anti-CD14 antibody [MEM-15], prediluted (ab157312)
- APC/Cy7® Anti-CD14 antibody [MEM-15], prediluted (ab194805)
- PE/Cy5® Anti-CD14 antibody [MEM-15] (ab232910)
- PE/Cy7® Anti-CD14 antibody [MEM-15] (ab234214)
- PerCP Anti-CD14 antibody [MEM-15] (ab269327)
- FITC Anti-CD14 antibody [MEM-15] (ab28061)
- Biotin Anti-CD14 antibody [MEM-15] (ab28118)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
Use 10µl per 100µl of whole blood or 106 cells in a suspension. |
Notes |
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Flow Cyt
Use 10µl for 106 cells. Use 10µl per 100µl of whole blood or 106 cells in a suspension. |
Target
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Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
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Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab60901 has not yet been referenced specifically in any publications.