APC Anti-CD15 antibody [MEM-158] (ab88032)
Key features and details
- APC Mouse monoclonal [MEM-158] to CD15
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgM
Overview
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Product name
APC Anti-CD15 antibody [MEM-158]
See all CD15 primary antibodies -
Description
APC Mouse monoclonal [MEM-158] to CD15 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Specificity
Reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. We have data to indicate that this antibody may not cross react with Pig. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Tissue, cells or virus corresponding to Human CD15. Human granulocytes
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Positive control
- Human blood cells
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General notes
The purified antibody (>95% by SDS-PAGE) is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 8.0
Preservative: 0.078% Sodium azide
Constituents: 0.2% BSA, Tris buffered saline
0.2% BSA (high-grade, protease free) -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The antibody is purified by size exclusion chromatography and adjusted for direct use following conjugation with Allophycocyanin. -
Clonality
Monoclonal -
Clone number
MEM-158 -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab88032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. ab154431 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
|
Target
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Relevance
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
Cellular localization
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
Alternative names
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
References (0)
ab88032 has not yet been referenced specifically in any publications.
