Key features and details
- APC Mouse monoclonal [MEM-158] to CD15
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgM
Product nameAPC Anti-CD15 antibody [MEM-158]
See all CD15 primary antibodies
DescriptionAPC Mouse monoclonal [MEM-158] to CD15
ConjugationAPC. Ex: 645nm, Em: 660nm
SpecificityReacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. We have data to indicate that this antibody may not cross react with Pig. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Species independent
Tissue, cells or virus corresponding to Human CD15. Human granulocytes
- Human blood cells
The purified antibody (>95% by SDS-PAGE) is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 8.0
Preservative: 0.078% Sodium azide
Constituents: 0.2% BSA, Tris buffered saline
0.2% BSA (high-grade, protease free)
Concentration information loading...
Purification notesThe antibody is purified by size exclusion chromatography and adjusted for direct use following conjugation with Allophycocyanin.
Our Abpromise guarantee covers the use of ab88032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
ab154431 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
ab88032 has not yet been referenced specifically in any publications.